Measuring the DNA content of cells is a well established method to monitor cell proliferation, cell cycle, and DNA ploidy. The BD CycletestTM Plus DNA reagent kit provides a set of reagents for isolating and staining cell nuclei from tissue specimens or cell suspensions, which can be a...
Multicellular tumor spheroids (MCTSs) can serve as in vitro models for solid tumors and have become widely used in basic cancer research and drug screening applications. The major challenges when studying MCTSs by optical microscopy are imaging and analy
Moreover, they were able to demonstrate the potential utility of the map and identify a potential candidate gene that stimulates growth and cell division, as well as a region associated with sex determination. In an age when high-throughput sequencing is becoming more accessible, their study/...
This step is critical as replication must occur once, and only once, per cell cycle to ensure genomic stability. As such, human cells have adopted several strategies for preventing origin re-licensing. These include elevated CDK activity during the latter half of the proliferative cycle, resulting...
The proteins encoded by 35 of these genes are involved in DNA repair and in some cases also in other aspects of the DNA damage response such as cell cycle arrest and apoptosis. The polymerase coded for by the 36th gene, XPV (POLH), is involved in bypass (rather than repair) of DNA ...
1 It is adapted from the single-cell PBAT method that we have applied for DNA methylation analysis of human preimplantation embryos.9 Using the method, we have demonstrated that not only blastocysts and cumulus cells, but also polar bodies, contribute to the SECM cfDNA.1 The contribution of ...
关键词 : 无细胞蛋白合成 ;细胞提取物 ;质粒 DNA 模板 ;线性 DNA 模板 DOI :10.13560/j.cnki.biotech.bull.1985.2022-0346 Research Progress of Cell Extracts and Template DNAs in Cell-free Protein Synthesis System XU Bo-nan FENG Jia ZHOU Jian-ting JIANG Jian-lan (Key Laboratory of Systems ...
(small EVs) maintains cellular homeostasis and avoids senescence-like cell-cycle arrest or apoptosis [41]. In particular, the inhibition of exosomes secretion has been found to activate the cytoplasmic inflammatory sensing machinery, cGAS/STING, owing to accumulation of nuclear DNA fragments in the ...
(Fig.2Bi). Given these scores,p-values were calculated for each arm using an appropriate test statistic and a t-distribution with 8 degrees of freedom (9 controls minus 1), and they were corrected for multiplicity across all arms in each sample using the Benjamini-Hochberg procedure (see ...
Viruses express viral transcriptional regulators (vTRs) that can modulate host gene expression, altering immune responses, apoptosis, differentiation, and cell cycle dynamics42. vTRs participate in extensive interactions with human proteins42,43,44, but less is known about the functional outcomes of th...