Moreover, N 6 -methyladenosine (m 6 A) methyltransferase WT1 associated protein (WTAP) binds to the m 6 A modified site of DLGAP1-AS1 and motivates its stability. Mechanistically, DLGAP1-AS1 sponged miR-299-3p through 3?-untranslated region (3?-UTR) binding, which in turn relieved the ...
Besides, overexpression of DLGAP1‐AS1 was also proved in HCC cell lines. Moreover, DLGAP1‐AS1 knockdown efficiently suppressed cell proliferation in HCC. Interestingly, miR‐486‐5p was predicted and validated to interact with DLGAP1‐AS1, while the level of miR‐486‐5p was significantly ...
Moreover, we elaborated that the cancerogenic effects of DLGAP1-AS1 in HCC cells could be effectuated via activating Wnt/尾-catenin pathway by positively regulating CDK8 and LRP6, downstream genes of miR-26a/b-5p. In conclusion, our results demonstrated the detailed molecular mechanism of DLG...
143B及正常成骨细胞hFOB1.19中DLGAP1-AS1的表达.将HOS细胞分为DLGAP1-AS1组和control组,分别转染DLGAP1-AS1过表达载体和对照空载体.Transwell实验和流式细胞术检测骨肉瘤细胞侵袭和细胞周期.双荧光素酶报告基因系统验证DLGAP1-AS1与miR-744-3p的靶向关系.qRT-PCR检测miR-744-3p表达.qRT-PCR和Western blotting检测...
MiR-515-5p could be sponged by DLGAP1-AS1 in GBM cells and to repress cell proliferation in GBM. Further, Rho-associated coiled-coil containing protein kinase 1 (ROCK1) and Nuclear factor erythroid-2 like 1 (NFE2L1) were confirmed as the target gene of miR-515-5p. Wnt signaling ...
Furthermore, miR-149-5p was confirmed to directly bind to TGFB2 and DLGAP1-AS1 could regulate the expression of TGFB2 signaling pathway via miR-149-5p in CRC. These new findings indicate that DLGAP1-AS1 knockdown inhibited the progression of CRC and enhanced the 5-FU sensitivity of CRC ...
Conclusion: LncRNA DLGAP1‐AS1 accelerated cell proliferation in GBM via targeting miR‐515‐5p/ROCK1/NFE2L1 axis and activating Wnt signaling pathway.Wang, ZixuanHan, YipengLi, QifengWang, BaochengMa, JieBrain & Behavior
The si-DLGAP1-AS1+ox-LDL group decreased cell death and Bax expression, increased Bcl-2 expression, decreased MDA and ROS levels, and increased SOD and CAT levels than in the si-NC+ox-LDL group. Down-regulation of DLGAP1-AS1 was targeted in order to promote miR-26a-5p expression. ...