lncRNA DLX6-AS1在恶性肿瘤中的研究进展 桂宇欣，孙杰克，石玉荣* 蚌埠医学院检验医学院，安徽 蚌埠 收稿日期：2023年3月10日；录用日期：2023年4月1日；发布日期：2023年4月18日 摘 要 长链非编码RNAs 表达具备时间特异性和空间特异性。DLX6-AS1是lncRNAs 家族中的一员，其表达水平在多种恶性肿瘤中发生...
DLX6-AS1 knockdown efficiency in macrophages, NC: Negative control; (C) Detection of the cell viability by MTT; (D) Detection of the key pyroptotic protein GSDMD-N expression by immunofluorescence staining. P<0.05 represents a significantly statistical difference; scale bar = 40 滋m; n=3. ...
结果发现lncRNA DLX6-AS1与miR-181b存在互补结合位点,双荧光素酶报告基因实验进一步验证了两者的靶向关系,且下调lncRNA DLX6-AS1可使miR-181b表达上调;在细胞功能实验中也发现上调miR-181b表达后Eca-109细胞增殖、迁移及侵袭能力...
lncRNA DLX6-AS1:长链非编码生长停滞特异性蛋白6反义RNA1;NUCKS1:核酪蛋白激酶和细胞周期蛋白依赖性激酶底物1;Cyclin D1:细胞周期蛋白D1;MMP:基质金属蛋白酶 双荧光素酶报告系统实验显示,lncRNA DLX6-AS1靶向结合miR-16-5p,miR-16-5p靶向...
The expression of DLX6-AS1, miR-204-5p and OCT1 in 56 GC patients and GC cell lines was quantified by qRT-PCR and western blotting. Furthermore, we performed in vitro functional assays to assess proliferation, invasion and migration of GC cells by knockdown of DLX6-AS1. The expression ...
目的:探讨长链非编码RNA(long non-coding RNA,lncRNA)生长停滞特异性蛋白6-反义RNA1(growth arrest specific protein 6-antisense RNA1,DLX6-AS1)对口腔鳞状细胞癌(口腔鳞癌)细胞增殖、迁移和侵袭的影响及作用机制。方法:实时荧光定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)检测口腔...
DLX6-AS1/miR-204-5p/OCT1 positive feedback loop promotes tumor progression and epithelial–mesenchymal transition in gastric cancer Gastric Cancer (2020) -4Comments doi: 10.1007/s10120-019-01002-1 issn: 1436-3291 issn: 1436-3305 pubmed: 31463827 ...
The aim of the present study was to identify the molecular mechanisms of the lncRNA known as DLX6 antisense RNA 1 (DLX6-AS1) in endometrial cancer. Microarray-based analysis was utilized to predict expression profile and possible function pattern of DLX6-AS1 and DLX6 in endometrial cancer, ...
The expression levels of IncRNA DLX6-AS1 in tissue samples and serum exosomes were quantified using real-time quantitative PCR (qRT-PCR). The clinical diagnostic value of IncRNA DLX6-AS1 for cervical cancer was evaluated using receiver operating characteristic (ROC) curve a...
心肌细胞H2O2凋亡氧化应激炎性反应目的探讨长链非编码RNA(LncRNA)生长停滞特异性蛋白6-反义RNA1(DLX6-AS1)靶向miR-103a-3p对H_(2)O_(2)诱导的心肌细胞损伤的影响.方法采用H_(2)O_(2)诱导H9C2细胞建立氧化损伤模型.将H9C2细胞随机分为正常对照(NC)组,H_(2)O_(2)组,si-NC+H_(2)O_(2)组,si-Lnc...