A method, computer system, and computer program product for determining N disulphide bond connections in a protein having 2N bonded sulphur atoms. Each sulphur atom is available for forming the N disulphide bond connections through use of an undirected graph having the 2N sulphur atoms as nodes ...
Protein disulphide isomerase (PDI) is now known to catalyse all of the reactions that are involved in native disulphide bond formation, but despite more than 40 years of study, its mechanism of action is still not fully understood. This review discusses recent advances in our understanding of ...
These regions are known to be important in protein-protein contacts [14], and short (2-8 amino-acid) protein loops or turns can be "pinned" in place by a disulphide bond [15], forming a surface structural motif held in a relatively fixed position by the disulphide bond, thus having a...
PROTEIN disulphide isomerase (PDI)1,2 is a highly abundant and ubiquitous eukaryotic protein that is essential for viability in yeast3,4. Although PDI is thought to catalyse disulphide bond formation and isomerization during protein biosynthesis, PDI has been found previously to have only moderate ef...
In earlier studies certain staggers between rod domain segments were shown to be favoured on the basis of ionic interactions and in the present studies one of these was also found to have a high potential for disulphide bond formation. 展开 ...
A. Two pairs of conserved cysteines are required for the oxidative activity of Ero1p in protein disulfide bond formation in the endoplasmic reticulum. Mol. Biol. Cell 11, 2833–2843 (2000). CAS PubMed PubMed Central Google Scholar Cabibbo, A. et al. ERO1-L, a human protein that ...
structure of Pex4p C105S,C146S is comparable with that of wild-type Pex4p, suggesting that the function of the disulphide bond in Pex4p is unlikely to be in aiding protein folding. Next, we determined whether the disulphide bond is indeed present in Pex4p in solu- tion, to rule out ...
We then demonstrate that SpoVD is a membrane-bound protein with two exposed redox-active cysteine residues. Structural modelling of SpoVD, based on the well characterized orthologue PBP2x of Streptococcus pneumoniae, confirmed that a disulphide bond can form close to the active site of the ...
Grains and grain boundaries in highly crystalline monolayer molybdenum disulphide The effects of biocidal treatments on metabolism in soil—VI. Fumigation with carbon disulphide Thioredoxin regulates the DNA binding activity of NF-kappa B by reduction of a disulphide bond involving cysteine 62....
A critical processing step in endothelin biosynthesis is the conversion of the intermediate "big endothelin" to its biologically active product catalysed b... AJ Turner,LJ Murphy - 《Biochemical Pharmacology》 被引量: 446发表: 1996年 Native disulphide bond formation in protein biosynthesis: evidence ...