Thus, BBX21 is targeted for 26S proteasome-mediated degradation in dark-grown Arabidopsis seedlings in a COP1-dependent manner. Moreover, we show that BBX21 binds to the T/G-box in the ELONGATED HYPOCOTYL 5 (HY5) promoter and directly activates HY5 expression in the light. Transgenic ...
Interestingly, we found that PUB22 and PUB23 interact with RPN6, the other subunit of the lid complex (RP) in the 26S proteasome. By performing detailed molecular and biochemical experiments, we here present that PUB22 and PUB23 directly tether ubiquitin to RPN6 for further degradation and ...
SMURF1 and SMURF2 directly target GLI1 for ubiquitination and proteasome-dependent degradationThe transcription factor GLI1 is the main and final effector of the Hedgehog signaling pathway, which is involved in embryonic development, cell proliferation and stemness. Whether activated through canonical or...
mRNA and protein level, which can become disrupted in cancer cells. For example, in response to phosphorylation events at the TAD region, c-Myc interacts with ubiquitin ligases FBW7 and SKP2 at MBI and MBII respectively, which promote its proteasomal degradation reducing its ...
reported that cells treated with MG-132, a proteasome inhibitor, exhibited a significant MMP-9 protein accumulation compared to its accumulation in the untreated controls, indicating that the degradation of the MMP-9 protein is in a proteasome-dependent manner. Moreover, SMURF1, an E3 ubiquitin ...