direct rna sequencing - sequence-specific (sqk-rna004) pack size 6 reactions stability shipped at 2–8°c long-term storage -20 – -30°c the warranty for this product is 3 months from receipt by the customer the direct rna sequencing kit (sqk-rna004) is used to prepare and ...
所用的试剂盒为Direct RNA Sequencing Kit (SQK-RNA004),首先准备poly(A)富集的mRNA (300ng/8ul) 或者总RNA(1ug/8ul) ,通过poly(T)引物反转录合成cDNA链(稳定mRNA),为RNA-cDNA加上测序接头,最后在MinION或PromethION芯片上进行测序(图11),建库总用时大约为2小时15分钟。互补cDNA链不会被测序,只是为了提升...
4. Gao Y, Liu X, Wu B, Wang H, Xi F, Kohnen MV, Reddy ASN, Gu L. Quantitative profiling of N6-methyladenosine at single-base resolution in stem-differentiating xylem of Populus trichocarpa using Nanopore direct RNA sequencing. Genome Biol. 2021....
基于Nanopore测序平台,不经反转录、无需扩增,直接读取全长转录本,无测序偏好性。可以检测RNA分子上的(m6A、m5C和假尿苷)等甲基化修饰位点;能准确分析可变剪接、融合基因和鉴定新异构体;此外,还可对Poly(A)尾长度进行相对准确的估算,还原真实RNA特征。
Direct RNA sequencing does not require PCR and has no sequencing GC preference Because the PCR process has GC preference, it is not easy to amplify sequences with too high or too low GC content. Therefore, short-read sequencing will introduce GC preference during database construction and sequen...
Nanopore direct RNA sequencing (DRS) is a powerful tool for RNA biology but suffers from low basecalling accuracy, low throughput, and high input requirements. We present DEMINERS, a novel DRS toolkit combining an RNA multiplexing workflow, a Random Forest-based barcode classifier, and an optimiz...
RNA modifications such as m6A methylation form an additional layer of complexity in the transcriptome. Nanopore direct RNA sequencing can capture this information in the raw current signal for each RNA molecule, enabling the detection of RNA modifications using supervised machine learning. However, exper...
What direct RNA nanopore sequencing can bring to research across numerous applications, with a worked example. Authors:Libby Snell, Director, RNA and cDNA Sample Technology (R&D), Oxford Nanopore, Daniel Garalde, Associate Director - Technology Markets, Oxford Nanopore ...
Parallel to these experimental advances, computational tools have evolved to address the limitations of traditional m6A detection methods. Direct RNA sequencing (DRS) technologies, such as those developed by Oxford Nanopore Technologies (ONT)31, have revolutionized the field by allowing the sequencing of...