之所以叫indirectELISA方法,是因为相对于directELISA,它引入了第二个抗体来进行检测,与directELISA不一样的是,它通过使用一抗能结合多个二抗,从而放大了检测灵敏度,就好像在一个黑暗的房间,directELISA只有一个50W的灯泡,而indirectELISA能接上两个50W的灯泡,这样房间的亮度是不一样的。
modification of antibody binding. Therefore, direct and indirect ELISAs are often used for qualitative tests. Another noncompetitive method is the sandwich ELISA. Here, antibodies are immobilized to trap antigens from crude extracts. This technique requires that the antigens have at least two ...
A direct and an indirect quantitative ELISA for antisperm antibody were compared using the spermatozoa and cellfree seminal fluid of 66 infertile males. The normal concentration of sperm binding immunoglobulin was ¤ 1.5 fg Ig per spermatozoon for the indirect seminal plasma assay and ¤ 1.5 fg Ig...
However, the indirect sandwich ELISA is perhaps the most viable in the food industry, while the direct ELISA finds more application in clinical settings. Using immobilized antigen probes, the indirect ELISA detect the presence of specific proteins (toxins, enzymes) or other microbial antigens in ...
Babesia divergens: an ELISA with soluble parasite antigen for monitoring the epidemiology of bovine babesiosis An enzyme linked immunosorbent assay (ELISA) for bovine babesiosis caused by Babesia divergens was developed to analyse the evolution of the serological st... A Chauvin,Monique L'Hostis,Alexi...
Comparison of modified direct agglutination and indirect haemagglutination tests for detection of Toxoplasma gondii antibodies in goats in Chennai Comparison of modified direct agglutination and indirect haemagglutination tests for detection of Toxoplasmn gondii antibodies in goats in Chennai. J. Vet. .....
The development and application of an indirect ELISA test for the detection of antibodies to bovine respiratory syncytial virus in blood serum We developed an indirect enzyme-linked immunosorbent assay (ELISA) for detection of serum antibodies to bovine respiratory syncytial virus. For evaluation ... ...
Ramakrishna N and VK Mehan Direct and indirect competitive monoclonal antibody-based ELISA of Aflatoxin B1 in groundnut. J. Mycotoxin Res. 1993; 9 (1): 53-63.Ramakrishna N, Mehan VK. Direct and indirect com- petitive monoclonal antibody-based ELISA of aflatoxin B1 in groundnut. Mycotoxin Res...
J. Henschel, and H. Burton. 1973. Comparison of milks processed by the direct and indirect methods of ultra-high-temperature sterilization. VI. Effects... AG Perkin,MJ Henschel,H Burton - 《Journal of Dairy Research》 被引量: 68发表: 1973年 Comparison of milks processed by the direct an...
Compared with two commercial indirect ELISA (i-ELISA) kits, our Dc-ELISA offered a higher positive detection rate in 165 clinical bovine serum samples. Conclusions A rapid, sensitive, and reliable serological diagnosis method was developed for M. bovis, which can facilitate M. bovis surveillance,...