IndirectELISA 由于受到directELISA方法的鼓舞,Lindström和Wager在1978年发表了indirectELISA方法5,之所以叫indirectELISA方法,是因为相对于directELISA,它引入了第二个抗体来进行检测,与directELISA不一样的是,它通过使用一抗能结合多个二抗,从而放大了检测灵敏度,就好像在一个黑暗的房间,directELISA只有一个50W的灯泡,而...
There was no statistically significant difference between results from the direct and indirect methods of the patients as a group. However, there was evidence of autospecificity in a small percentage of males who had elevated levels of antisperm antibody by the direct assay that was not detected ...
Using immobilized antigen probes, the indirect ELISA detect the presence of specific proteins (toxins, enzymes) or other microbial antigens in food samples. The sandwich ELISA employs a dual antibody system, the first antibody is embedded in the probe or well, and the second is linked to an ...
The data was analyzed using SigmaPlot (Systat Software, Inc., San Jose, CA, USA), Normal distribution was established using the Shapiro-Wilk Normality Test, and equal variance by the Brown-Forsythe test. Difference between groups was established using unpaired 2-tailed Student's t-test (for ...
resulted Hp+ve infection, 5 of these showed sPGII (mean value 20.4 range 11-30) greater than normal values; there was significative difference (p = 0.05) between Hp+ve patients and Hp-ve patients. Conclusion: serum pepsinogen II levels remain high in Hp+ve patients in comparison with Hp-...
The disease is endemic in Ardabil, East Azarbaijan, Bushehr and Fars provinces. This study was performed with the aim of evaluation and comparison of direct agglutination test (OAT), indirect immunofluorescent antibody (IFAT) and ELISA in sero-diagnosis of visceral leishmaniasis that is following ...
Comparison of monoclonal antibody-based ELISA for triazophos be- tween the indirect and direct formats. Food Agric. Im- munol., 19(1):49-60. http://dx.doi.org/10.1080/09540100801933454Jin, R., Gui, W., Guo, Y., Wang, C., Wu, J. and Zhu, G. 2008. Comparison of monoclonal ...
341.6: Direct and Indirect Effects of an Educational And Communication Skills Intervention to Increase Donor Designation in Latinx Communities: Promotoras de DonacióNdoi:10.1097/01.tp.0000887092.94785.1dHeather GardinerCollege of Public Health, Temple University, Philadelphia, PA, United StatesLaura A ...
In both the direct and the indirect transmission study, the pigs were monitored for two weeks, with respect to the excretion of Salmonella in faeces and the presence of antibodies in serum. Further sampling to demonstrate presence of Salmonella was carried out at necropsy at the end of the tri...
difference between ELISPOT and enzyme-linked immunosorbent assay (ELISA) (supernatant evaluation of cytokines) is that in the ELISPOT assay, cytokines are captured immediately on secretion from cells and are not subject to dilution, breakdown, or being used up by other cells. Another core attribute...