(also called competitive indirect) ELISAs are based on the inhibition of the reaction of enzyme-labeled antibodies and theimmobilized antigenby free antigen present in the test or calibration sample. The amount ofenzyme immobilizedon the solid phase is inversely proportional to the amount of free ...
A direct and an indirect quantitative ELISA for antisperm antibody were compared using the spermatozoa and cellfree seminal fluid of 66 infertile males. The normal concentration of sperm binding immunoglobulin was ¤ 1.5 fg Ig per spermatozoon for the indirect seminal plasma assay and ¤ 1.5 fg Ig...
The development and application of an indirect ELISA test for the detection of antibodies to bovine respiratory syncytial virus in blood serum We developed an indirect enzyme-linked immunosorbent assay (ELISA) for detection of serum antibodies to bovine respiratory syncytial virus. For evaluation ... ...
The disease is endemic in Ardabil, East Azarbaijan, Bushehr and Fars provinces. This study was performed with the aim of evaluation and comparison of direct agglutination test (OAT), indirect immunofluorescent antibody (IFAT) and ELISA in sero-diagnosis of visceral leishmaniasis that is following ...
To determine the potential risk of transfusion malaria at the Hospital Militar Central in Bogota, Colombia, sera from 3114 blood donors were tested for malaria antibodies by the indirect ELISA technique. Positive results were found in 8路6 per thousand of the serum samples using P. falciparum ...
Background: Helicobacter pylori stool antigen test (HpSA) is a usefull,non-invasive tool for diagnosis and for the check of the eradication after therapy, in Helicobacter pylori (Hp) infection. Serum pepsinogen II (sPGII) levels were found increased during Hp infection mostly due to the antrum...
Comparison of modified direct agglutination and indirect haemagglutination tests for detection of Toxoplasma gondii antibodies in goats in Chennai Comparison of modified direct agglutination and indirect haemagglutination tests for detection of Toxoplasmn gondii antibodies in goats in Chennai. J. Vet. .....
Lalle, and E. Pozio, 2014: Indirect versus direct detection methods of Trichinella spp. infection in wild boar (Sus scrofa). Parasit. Vectors 7, 171.Gomez-Morales, M.A., Ludovisi, A., Amati, M., Bandino, E., Capelli, G., Corrias, F., Gelmini, L., Nardi, A., Sacchi, C.,...
This setup was used in both a direct and indirect detection system that did not require further addition of dyes or chromophores. The setup was able to carry out direct detection of the antibody as well as conduct a competitive in direct assay to determine the presence of the target antigen ...
Detection of parasitesDIG-ELISA, Dot-ELISA, and indirect ELISASeroprevalence of antibody produced in host serum against secretory or excretory metabolites of parasites (e.g., liver flukes in cattle) Medicine industriesQuality control for antibioticsKanamycin, sulfonamides, fluoroquinolones ...