What is digital PCR used for? In one sentence, digital PCR is used to detect rare sequences, copy number variations and microbial targets; to quantify of rare mutations, including from liquid biopsies; for single-cell analysis; measurement of viral loads; to analyze gene expression; and to qu...
The aim of this study was to isolate and analyse single cells using a novel platform, the PanelChip™ Analysis System, which includes 2500 microwells chip and a digital real-time polymerase chain reaction (dqPCR) assay, in comparison with a standard PCR (qPCR) assay. Through the serial ...
Droplet-based digital PCR system for detection of single-cell level of foodborne pathogensDroplet-based digital polymerase chain reaction (ddPCR)Foodborne pathogensE. coli O157:H7Salmonella cellsSingle-cell analysisQuantitative analysisRecently, foodborne pathogen is a common and distressing disease around ...
Positive results showing the detection of specific transcripts using fixed intact cells for direct RT-PCR analysis were already published for a microfluidic helical tube reactor25. Figure 1 confirms these data and shows a specific detection of both one cell-equivalent SiHa-RNA and single unfixed and...
Digital PCR (dPCR) provides accurate and reliable DNA measurement without the need for standard curves, making it an invaluable tool for cancer researchers. The high sensitivity and accuracy of this direct quantification technique allow for the consistent detection of low-abundance mutations and the ...
Digital PCR (dPCR) is a leading technology for detection and quantification of rare mutations and target sequences. Overcome challenges with other ddPCR and dPCR platforms and leverage the full power of dPCR with the Applied Biosystems QuantStudio Absolute Q Digital PCR System, featuring: ...
Digital PCR (dPCR) is a leading technology for detection and quantification of rare mutations and target sequences. Overcome challenges with other ddPCR and dPCR platforms and leverage the full power of dPCR with the Applied Biosystems QuantStudio Absolute Q Digital PCR System, featuring: Broad ap...
11 Rausch, C. et al. Double Drop-Off Droplet Digital PCR: A Novel, Versatile Tool for Mutation Screening and Residual Disease Monitoring in Acute Myeloid Leukemia Using Cellular or Cell-Free DNA. J Mol Diagn23, 975-985, doi:10.1016/j.jmoldx.2021.05.001 (2021). ...
Digital PCR (dPCR) is a refinement of PCR where DNA is subject to PCR for amplification of the template, then the sample undergoes fluorescent detection and sequence-specific alleles can be directly counted. This method offers a highly sensitive quan
While we use ddPCR for AAV titration, there are many applications for the technology. Droplet digital PCR is well suited for the detection of low copy numbers. For this reason, it can be used in the detection of rare sequences and single cell analysis. It is also being used in the detec...