(2012) Differential RNA sequencing (dRNA-Seq): deep- sequencing-based analysis of primary transcriptomes. In: Harbers,M. and Kahl,G. (eds) Tag-Based Next Generation Sequencing. Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany, pp. 109-121....
A variety of so-called RNA sequencing (RNA-seq) methods have been applied to investigate eukaryotic and prokaryotic transcriptomes. Here, we describe a differential RNA-seq (dRNA-seq) approach for the selective analysis of primary transcripts in the cell. The method is based on a differential ...
sequana/rnaseq This is is theRNA-seqpipeline from theSequanaproject Overview: RNASeq analysis from raw data to feature counts Input: A set of Fastq Files and genome reference and annotation. Output: MultiQC and HTML reports, BAM and bigwig files, feature Counts, script to launch differential ...
Differential RNA sequencing (RNA-seq) of wild-type V. cholerae and a locked low-cell-density QS-mutant strain identified 7,240 transcriptional start sites with ∼ 47% initiated in the antisense direction. A total of 107 of the transcripts do not appear to encode proteins, suggesting they ...
RNA sequencing (RNA-seq) has been rapidly adopted for the profiling of transcriptomes in many areas of biology, including studies into gene regulation, development and disease. Of particular interest is the discovery of differentially expressed genes across different conditions (e.g., tissues, perturb...
Following deep sequencing, RNA molecules and antisense RNAs. We recently developed a the obtained short sequences (’reads’) are mapped onto a differential RNA-seq (dRNA-seq) method which in addition to reference genome for further data analysis. To maintain the above, yields information as ...
无论是传统的多细胞转录组测序(bulk RNA-seq)还是单细胞转录组测序(scRNA-seq),差异表达分析(differential expression analysis)是比较两组不同样本基因表达异同的基本方法,可获得一组样本相对于另一组样本表达显著上调(up-regulated)和下调的基因(down-regulated),从而可进一步研究这些差异表达基因的功能,包括富集的通路...
Recent developments in RNA-sequencing (RNA-seq) technology have led to a rapid increase in gene expression data in the form of counts. RNA-seq can be used ... H Wu,C Wang,Z Wu - 《Biostat》 被引量: 201发表: 2013年 FDM: a graph-based statistical method to detect differential transcri...
Library construction and sequencing were performed by OMICS Solutions (Santiago, Chile). The same RNA-Seq data were used to perform the de novo assembly and to assess gene expression. Short read, quality control and de novo assembly This part of the analysis was similar to that described in ...
Recent advances in high-throughput cDNA sequencing (RNA-seq) can reveal new genes and splice variants and quantify expression genome-wide in a single assay. The volume and complexity of data from RNA-seq experiments necessitate scalable, fast and mathema