Herein, we evaluated and compared three different ready-to-use direct cell radiolabeling synthons, [89Zr]Zr-DFO-Bn-NCS, [89Zr]Zr-Hy3ADA5-NCS, and [89Zr]Zr-Hy3ADA5-SA for PET imaging-based trafficking of white blood cells (WBCs) and stem cells (SCs) up to 7days in athymic nude...
Other isoforms of dystrophin are expressed in white blood cells, neurons , and the Schwann cells that wrap neurons with insulation. Thus, it is difficult to speak of "the" dystrophin gene because the alternative splicing of noncontiguous pieces of RNA produces a variety of Figure 3. Gene ...
aPeripheral blood mononuclear cells were isolated from healthy controls and patients at different post-trauma time points and labeled with anti-human antibodies against CD3 and CD56. Representative dot plot of CD3 and CD56 staining (gated on PBMCs of patients or healthy controls). The absolute numb...
Regulation of hierarchical clustering and activation of innate immune cells by dendritic cells. An early granulomatous response, characterized by collections of white blood cells at foci surrounding pathogens, occurs after infection by many intracellu... SJ Kang,HE Liang,B Reizis,... - 《Immunity...
were labeled w Figure 3 Accutase cleaved surface FasL proteins. (a,b) RAW264.7 cells were incubated with either accutase or EDTA cell detachment buffer for 30 min. The supernatants and lysates in both groups were collected and concentrated. The FasL proteins in the supernatant and lysates were ...
We have previously described how labeled proteins and other macromolecule tracers may cross the nasal epithelia via paracellular or transcellular transport to reach the underlying lamina propria of the nasal respiratory and olfactory regions, after which they may (i) be absorbed into nasal blood ...
Monocytes are also key effectors of the innate immune response to pathogens and contribute to recruitment of T-cells at sites of infection[21]. In turn, the activated T cells assist other white blood cells in immunologic processes, including maturation of B cells into plasma cells and memory B...
White blood cells were isolated by erythrocyte lysis. RNA was isolated using the RNeasy kit (Qiagen) according to the manufacturer's instructions. 2.2. cDNA synthesis cDNA was made using SuperScript II or SuperScript III (Life Technologies) according to the manufacturer's instructions. Briefly, 1...
The K-means clustering method clustered all 5060 participants into three clusters, which can be seen in Fig. S2. Cluster 1 was labeled as the “low-level exposure group”, Cluster 2 as the “middle-level exposure group”, and Cluster 3 as the “high-level exposure group”. Table S2 and...
cells), ET (n = 5 cells), PV (n = 6 cells). HSPCs at day 3, WT (n = 40 cells), ET (n = 45 cells), PV (n = 74 cells).i, Representative BM Z-stacks 3 days after transplantation intoNes-GFPmice (with genetically labeled HSC-niche-forming cells32). ...