DNA extraction methods capable of removing inhibitory substances of environmental origin and recovering the DNA are decisive for the efficiency of PCR. This study aimed to compare the efficiency of different DNA extraction methods for the search by Cryptosporidium oocysts in water samples by molecular ...
这几种豆粉基因 组DNA提取方法都具有操作简单、耗时短、利于快速检测的优点。 豆粉;基因组DNA;提取;聚合酶链式反应 S565.1A Comparison of Different Extraction Methods of Genomic DNA from Soybean Powder Xu Weili Ma YingDu MingWang JiaqiLi Qiming 四川省应用基础研究(2009JY0101);哈尔滨工业大学优秀青年教师...
Comparison of three methods of DNA extraction from human bones with different degrees of degradation. Int J Leg Med 2012;126:173e8.Jakubowska J, Maciejewska A, Pawłowski R. Comparison of three methods of DNA extraction from human bones with different degrees of degradation. Int J Legal Med...
Comparison of Six DNA Extraction Methods for Recovery of Fungal DNA as Assessed by Quantitative PCR The detection of fungal pathogens in clinical samples by PCR requires the use of extraction methods that efficiently lyse fungal cells and recover DNA suit... DN Fredricks,C Smith,A Meier - 《Jo...
of DNA extracted by SDS method was the most amoung the four methods;when the quality of Staphylococcus Aureus' DNA diluted to 1.975×10-4 pg,SDS method can still detect the extractives by PCR. SDS method was stable and economic and was an ideal extraction of Staphylococcus Aureus'DNA. ...
Our ongoing study is aiming to test the impact of three different age of faeces samples and two DNA extraction methods on the genotyping success of Sumatran tiger (Panthera tigris sumatrae). Samples from zoos in Germany are separated into three different part (fresh, 1 week and 1 month). ...
关键词:乳品废水;活性污泥;DNA;提取;PCR 中图分类号:X703 文献标识码:A ComparisonofDifferentDNAExtractionMethods fromDairyWastewaterActivatedSludge WenHongyu,WangLu,YangLiu,HuiXingxing,TongHe,YangYifan (CollegeofLifeSciences,XuzhouNormalUniversity,Xuzhou221116,China) Abstract:InorderextracttotalDNAfromdairywastew...
DNA extraction methods consists of three common steps: lysis, purification, and DNA recovery, with lysis being the most critical step (Barbosa, Nogueira, Gadanho, & Chaves, 2015). In addition, DNA can be extracted using either a conventional method or a commercial extraction kit. A conventional...
samples were collected from multiple regions of the gastrointestinal tract and characterized using culture-independent sequencing methods. Results demonstrated that seemingly benign husbandry factors can interact to induce profound changes in the composition of the microbiota present in certain regions of the...
Material and Methods Study group and sampling Pregnant women were enrolled in this study during the first period of spontaneous onset of labor or up to 24 h before elective caesarean section with intact amniotic membranes. They were enrolled at the delivery ward of the Women’s Clinic of Tart...