GLYCOSYLTRANSFERASE, METABOLIC-ADJUSTED ESCHERICHIA COLI, AND METHOD FOR SYNTHESIZING QUERCETIN 3,7-DI-O-RHAMNOSIDEThe present invention relates to a method for producing quercetin 3,7-di-O-rhamnosidemade in plants using Escherichia coli, and more specifically, to metabolic-adjusted Escherichia coli ...
favone di-C-glycosidesubstrate selectivityHerein,we discovered an efcient favone 6-C-glycosyltransferase GcCGT from the medicinal plant Gentiana crassicaulis.GcCGT could catalyze consecutive two-step 6-C/4′-O-glycosylation of favonoids.Homology modeling and site-directed mutagenesis yielded mutant ...