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The exchange reaction was started upon addition of 20 mM EDTA or 0.5 μM Dbs DH–PH as positive controls, and 5 μM or 50 μM Bcr-Abl DH–PH and DH domains and immediately measured at the SpectraMax M5 plate reader (Molecular Devices). The single-wavelength measurement was ...
The developed blue color in the reaction was measured at 560 nm. One unit of SOD activity was defined as the amount of enzyme required to inhibit the reduction of NitroBlueTetrazolium by 50% and activity was expressed as units per mg protein. GSH level. GSH, a major intracellular ...
Da Won ChaeBaekjin KimYoung Gyu KimElsevier B.V.Journal of Industrial & Engineering ChemistryKang, E-S.; Chae, D.W.; Kim, B.; Kim, Y.G. Efficient preparation of DHMF and HMFA from biomass-derived HMF via a Cannizzaro reaction in ionic liquids. J. Ind. Eng. Chem. 2012, 18, 174...
The platform was operated in a multiple reaction monitoring (MRM) in negative mode using an electrospray ionization (ESI) source. The optimized transition of amino acid metabolites is listed in Table S3 (Supplementary Materials). The injection volume was 2 µL. Mobile phase A was 10 mM ...
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