These observations inspired the thought that the lipid raft domain in the membrane is the domain in the liquid ordered phase, and that a strong correlation exists between the molecules recovered in the DRM fraction and those partitioned into raft domains in the membrane. Here we have isolated ...
Studies in cell lines have shown that -secretase is partially localized to lipid rafts, which are detergent-resistant membrane microdomains enriched in cholesterol and sphingolipids. Here, we studied -secretase in detergent-resistant membranes (DRMs) prepared from human brain. DRMs prepared in the ...
This set of proteins was used as a “plasma membrane protein database” for a comparison with DRM proteins. Isoelectric Point— The theoretical isoelectric points of all the proteins identified range from 4 to 12 and are mainly represented in two groups: an acidic group (pH 5–7) and a ...
Although Sig-1Rs are predominantly expressed at endoplasmic reticulum (ER) subdomains apposing mitochondria [i.e., the mitochondria-associated ER membrane (MAM)], they dynamically change the cellular distribution, thus regulating both MAM-specific and plasma membrane proteins. However, what determines ...
In addition, DRMs represent a minor and transient component of the total membrane surface. The classical method of isolating DRM-enriched fractions is by flotation of cell extracts, prepared in cold detergents such as Triton X-100 or under detergent-free conditions, in a sucrose density gradient ...
existence of DRM in intact cells [9]. It is also possible that DRM preparations do not reflect the exact composition of the membrane rafts in intact cells, however, the purification of such domains has led to significant progress in understanding the functional architecture of biological...
Table 4Detergent resistant membrane (DRM) fatty acids composition of HT29-dx cells Full size table Both DHA and EPA were integrated in DRM phospholipids even if with lower efficiency compared to their incorporation in whole cell lipids (Tables3and4); in particular in HT29-dx cells they were ...
Table 4 Detergent resistant membrane (DRM) fatty acids composition of HT29-dx cells Full size table Both DHA and EPA were integrated in DRM phospholipids even if with lower efficiency compared to their incorporation in whole cell lipids (Tables 3 and4); in particular in HT29-dx cells they...
PKC activation strongly increased its association with membranes (from 2% to 75%), causing its enrichment within DRM; the substrate GAP43, enriched in DRM, remained membrane associated, but its proportion in DRM dramatically decreased (from about 40% to 2.5%), suggesting its shift from raft to...
Detergent resistant membrane domains (DRM) can for the first time be visualised in the context of their native environment (i.e. attached to a cell) using correlative fluorescence and electron microscopy (CFEM). DRMs found on whole mounted colorectal cancer cells have a size range that is ...