Protein solubility of TM-Ubc9ts was determined in transiently transfected HEK293 cells after treatment with NP-40 to solubilize membrane proteins (soluble fraction) and then RIPA (insoluble fraction). (A) Levels of protein were analyzed via western blot after centrifugation and resuspension in the ...
Characterization of 230-kD bullous pemphigoid antigen associated with the detergent-insoluble fraction of cultured keratinocytes. J Invest Dermatol. 1991 Jan; 96 (1):139–143.Thacher SM, Hefti PL: Characterization of 230-kD bullous pemphi- goid antigen associated with the detergent-insoluble fraction...
Identification of members of the annexin family in the detergent-insoluble fraction of rat Morris hepatoma plasma membranes 来自 万方 喜欢 0 阅读量: 40 作者:JG Clifton,MK Brown,F Huang,X Li,W Reutter,W Hofmann,DC Hixson,D Josic 摘要: For proteomic analysis, plasma membranes of rat ...
However, we failed to detect any increase in Yes activity in the DIM fraction (Fig.6). In contrast, Yes kinase activity in DS showed the same kinetics as that of whole cell lysate, indicating that the activated Yes was mostly located in the DS. That Yes activity in DIM was lost or ...
Since many excellent reviews are written on the membrane lipid dynamics of this microdomain, signal transduction, and neuronal glycolipids, we review on the characterization of the raft proteins recovered in the detergent-insoluble low-density fraction from rat brain. Special focus is addressed on the...
Here we tested the hypothesis that PTMs on neuronal tubulins selectively accumulate in a pathological protein fraction in AD. We used new software, P-MOD, to identify comprehensively and map PTMs using mass spectral data from soluble (normal) and detergent-insoluble (pathological) protein ...
Dans cette étude, les auteurs décrivent un protocole abrégée seule étape fractionnement pour l’enrichissement des agrégats de protéine insoluble dans le détergent du cerveau humain de post-mortem. Le détergent ionique N-lauryl-sarcosine (sarkosyl) solubilise efficacement les protéines nat...
The presence of these proteins, which all contribute to the generation of Aβ, indicates that the DIG fraction is probably where the intra-membranous cleavage of APP occurs. 展开 DOI: 10.1038/nm0698-730 收藏 引用 批量引用 报错 分享 全部来源 求助全文 全文购买 Nature Springer www.socolar.com ...
Protein in each fraction was quantitated by scanning densitometry of the gel. As shown in Table I, although the protein could be pelleted when liposomes were not extracted with detergent, the protein was efficiently solubilized by Triton X-100. Thus, the detergent insolubility of bovine and ...
In MDCK epithelial cells, cell contact at confluency initiates a protracted process of morphogenesis during which several proteins known to bind the cytoskeleton become progressively associated with the detergent-resistant cell fraction and distributed to their characteristic polarized domains. Using extraction...