Protein solubility of TM-Ubc9ts was determined in transiently transfected HEK293 cells after treatment with NP-40 to solubilize membrane proteins (soluble fraction) and then RIPA (insoluble fraction). (A) Levels of protein were analyzed via western blot after centrifugation and resuspension in the ...
These results indicate that during nucleotide excision repair, nuclear binding of PCNA precedes that of p21 protein, and suggest that temporal association of p21 with the detergent-insoluble fraction is coincident ...
Identification of members of the annexin family in the detergent-insoluble fraction of rat Morris hepatoma plasma membranes 来自 万方 喜欢 0 阅读量: 40 作者:JG Clifton,MK Brown,F Huang,X Li,W Reutter,W Hofmann,DC Hixson,D Josic 摘要: For proteomic analysis, plasma membranes of rat ...
Despite the widely recognized importance of neutral detergent-soluble carbohydrates (NDS) in the early stages of forage digestion, little is known about their digestion kinetics because most in vitro methods have studied the disappearance of insoluble cell wall components. However, knowledge of the ...
Since many excellent reviews are written on the membrane lipid dynamics of this microdomain, signal transduction, and neuronal glycolipids, we review on the characterization of the raft proteins recovered in the detergent-insoluble low-density fraction from rat brain. Special focus is addressed on the...
Here we tested the hypothesis that PTMs on neuronal tubulins selectively accumulate in a pathological protein fraction in AD. We used new software, P-MOD, to identify comprehensively and map PTMs using mass spectral data from soluble (normal) and detergent-insoluble (pathological) protein ...
6 , 12 , 13 , 14 une des méthodes plus courantes consiste à l’extraction séquentielle des homogénats de tissus avec des tampons et des détergents d’accroître la rigueur, suivi par ultracentrifugation pour partitionner les fractions solubles et insolubles. Un fractionnement séquentiel...
Also present in the DIG fractions are the endo-proteolytic fragments of presenilin-1 (PS1) and APP. The presence of these proteins, which all contribute to the generation of A, indicates that the DIG fraction is probably where the intra-membranous cleavage of APP occurs. 展开 ...
Disruptors of cytoskeletal filaments were relatively ineffective in the postconfluent epithelial monolayers, although use of disrupting agents implicated actin as the cytoplasmic filament that tethers insoluble GAPDH. Catalytic activity could be demonstrated in the insoluble fraction of GAPDH from postconfluent...
Presents a study which investigated the intracellular compartmentalization of amyloid beta-protein by using human neuroblastoma SH-SY5Y cells which were fractionated and the amyloid beta-protein content in each fraction was quantitated by an enzyme-linked immunosorbent assay (ELISA). Focus research of am...