Here we report that in the brain, the principal compartment in which Aβ resides is a detergent-insoluble glycolipid-enriched membrane domain (DIG). Also present in the DIG fractions are the endo-proteolytic fragments of presenilin-1 (PS1) and APP. The presence of these proteins, which all ...
Protein solubility of TM-Ubc9ts was determined in transiently transfected HEK293 cells after treatment with NP-40 to solubilize membrane proteins (soluble fraction) and then RIPA (insoluble fraction). (A) Levels of protein were analyzed via western blot after centrifugation and resuspension in the ...
Here we report that in the brain, the principal compartment in which A resides is a detergent-insoluble glycolipid-enriched membrane domain (DIG). Also present in the DIG fractions are the endo-proteolytic fragments of presenilin-1 (PS1) and APP. The presence of these proteins, which all ...
préparer 40 µg échantillons totales homogénats (TH-S), sark-soluble (S1), des fractions (P2) sark insoluble dans 1 X Laemmli SDS-page échantillon solution tampon 5 mM PTCE 7. Incuber les échantillons à 95 ° C pendant 5 min. Gel d’échantillons charge sur un préfabriqué ...
A GPI-anchored 80-kD protein was found to be the major component of detergent-insoluble complexes, prepared from fetal porcine small intestine, constitutin... Danielsen,M E. - 《Journal of Cell Biology》 被引量: 256发表: 1995年 加载更多来源...
(12) reported for the first time the isolation of Triton X-100-insoluble fractions from tobacco plasma membrane. Mongrand et al. (13) provided a detailed analysis of the lipidic composition of such a detergent-resistant fraction indicating that it is highly enriched in a particular species of ...
Prescription omega-3 fatty acid-induced prevention of impairment of memory learning in amyloid-beta-infused rats associates with the decrease of amyloid-beta levels in detergent-insoluble membrane fractions... H Michio,T Ryuichi,K Masahiro,... - 《Alzheimers & Dementia》 被引量: 0发表: 2011年 ...
The multiple membrane-spanning protein bacteriorhodopsin was incorporated into SCRLs. After exposure to Triton X-100 or mock extraction, solubilized and insoluble material were separated by ultracentrifugation. Protein was collected by trichloroacetic acid precipitation from supernatant and pellet fractions, ...
We show here that, unlike LAP2β, LAP2 behaved like a nuclear non-membrane protein in subcellular fractionation studies and was localized throughout the nuclear interior in interphase cells. It co-fractionated with LAP2β in nuclear lamina/matrix-enriched fractions upon extraction of ...
For this method, soluble materials have higher retention than membranes, but the distinction between soluble and insoluble materials will depend on the size fractionation range of the gel matrix. Another criterion is sedimentation in sucrose gradients, the membrane state being recovered at the bottom ...