Badizadegan, K, Dickinson, BL, Wheeler, HE (2000) Heterogeneity of detergent-insoluble membranes from human intestine containing caveolin-1 and ganglioside G(M1). Am J Physiol Gastrointest Liver Physiol 278: pp. G895-G904Badizadegan K, Dickinson BL, Wheeler HE, Blumberg RS, Holmes RK, ...
membranes (DRMs). We stress the fact that DRMs are artifactual in nature and should not be equivocated to lipid rafts, their usefulness being limited to assigning raft-association potential most convincingly when changes in DRM composition are induced by biochemically/physiologically relevant events. ...
Detergent solubility of an otherwise detergent-insoluble protein after cholesterol depletion with methyl-β-cyclodextrin can therefore serve as an additional criterion for raft association. However, the composition of DRMs may only imperfectly reflect the association of membrane components with lipid rafts ...
Selective solubilization of proteins and phospholipids from red blood cell membranes by nonionic detergents. Each detergent-insoluble ghost residue appeared by electron microscopy to be a filamentous reticulum with adherent lipoid sheets and vesicles. The residues contained most of the membrane sphingolipids...
Proteins were then transferred onto nitrocellulose membranes and stained with Revert 700 Total Protein Stain (Li-Cor) and imaged at 700 nm to detect total protein. After reversing the Revert Total Protein stain and rinsing in double distilled water, the blots were blocked in 1% milk/TBST for 1...
Incuber les membranes en solution d’anticorps primaire pendant la nuit à 4 ° C sous agitation circulaire. Rincer la membrane au SCT/T pour 3 x 15 min. Sonde la membrane avec une dilution de 1/20 000 de la fluorescent proche infrarouge secondaires anticorps marqués en BB/T pendant ...
Detergent-insoluble membrane fragments that are rich in sphingolipid and cholesterol can be isolated from both cell lysates and model membranes. We have pr... SN Ahmed,DA Brown,E London - 《Biochemistry》 被引量: 1307发表: 1997年 Polymer/surfactant interaction—Its relevance to detergent systems ...
After centrifugation for 20 h at 100,000 × g, a ring of Triton X-100-insoluble membranes was recovered at the 30–35% interface, diluted in buffer A, and centrifuged for 4 h at 100,000 × g. The pellet was resuspended in buffer A, and protein concentrations were determined using the...
The protein associated with detergent-insoluble membranes derived from SCRLs after extraction. This result showed directly that interactions between PLAP and lipids were sufficient for detergent insolubility of the protein; no other proteins were required. Based on further liposome studies on the lipid ...
Sphingolipid and cholesterol-rich Triton X-100-insoluble membrane fragments (detergent-resistant membranes, DRMs) containing lipids in a state similar to t... KA Melkonian,AG Ostermeyer,JZ Chen,... - 《Journal of Biological Chemistry》 被引量: 1498发表: 1999年 Luminescent probes for detergent ...