Determination of air-borne pyrogens by the in vitro pyrogen test (IPT) based on human whole blood cytokine response Introduction: The normal intravenous human immunoglobulin is a sterile liquid or lyophilized preparation, with immunoglobulins, especially the immunoglobul... I Kindinger,S Fennrich,B ...
These results emphasize the potential of using bovine whole blood for highly sensitive endotoxin testing. As a perspective, currently ongoing research aims to show whether the assay is also capable of detecting nonendotoxin pyrogens. 展开 DOI: 10.1111/jvp.12148 被引量: 7 ...
Bacterial endotoxins, when they enter the human bloodstream, can act as powerful pyrogens that lead to fever, inflammation, and in severe cases, septic shock. Gram-negative bacteria, which can be found everywhere in the environment, contain endotoxins in their outer membrane. The LAL assay, whic...
Difficulties in detecting LPSs in human fluids were identified very early on. The Limulus Amoebocyte Lysate or LAL method, described below, notably failed to detect the passage of LPS molecules through the membranes in dialysis tanks used to filter the blood of patients with kidney failure [38]....