Another atmospheric pressure ionization method is direct analysis in real time (DART), which uses the plasma-based technique. The ionization process involves an interaction between the analyte molecule and a gas (N2, Ne or He) that has been electrically excited to interact directly with the ...
dereplication; (2) MDF of resulting accurate mass full-scan raw data for a targeted identification of selected known natural products. In particular, MDF is a data-mining technique that can be applied after acquisition, permitting the reevaluation of data when future compounds of interest are ...
Agar overlay technique (A), agar plug technique (B), and dereplication of a collection of unknown antibiotic-producing actinomycetes (n = 60) using the ARP (C). In one step, it is possible to weed out >50% of known antibiotic-producing actinomycetes. See also Figures S2 and S3. The se...
Concentrations of IL-6, TNF-α, and MCP-1 in the collected cell culture supernatants were determined by a sandwich type ELISA technique using the Ready-Set-Go! Kit following the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific®, Bleiswijk, The Netherlands) [50]. 3.9. HPLC-...
Pinnatoxin G (PnTX-G) is a marine toxin belonging to the class of cyclic imines and produced by the dinoflagellate Vulcanodinium rugosum. In spite of its strong toxicity to mice, leading to the classification of pinnatoxins into the class of “fast-actin
Currently, this technique is widely used, such as in the study of forensics [43], food chemistry [44], environmental science [45], plant science [46], and others [47,48]. In natural product research, on the basis of CLMN, the feature-based MN (FBMN) [42], ion-identifying MN (...