Imperfect bins are a challenge for all studies that attempt to genomically resolve complex ecosystems. However, if they can be obtained, the value of high-quality draft genomes is enormous. Different single algorithm methods not only generate different numbers of bins, but the genome content can ...
Using either a low copy plasmid or integration of the gene into the bacterial chromosome, we control resistance gene copy number. Expression is further regulated through the use of two promoter systems differing in strength. To our knowledge, no other descriptions of such a comprehensive tool in ...
.Meanwhile,thediscoveryofnewnaturalcom-poundsisofgreatsignificancefordrugexploitationanddevelopment.Althoughtwo-dimensionalliquidchromatography(2DLC)systemsexpandthepeakcapacityandimproveselectivityandresolution,interpretingthepost-processingdataistediousandtime-consuming.Inthisstudy,anoff-linetwo-dimensionalliquid...
Indeed, with the large and increasing number of NPs (estimated at 600,000), redis- covery was commonplace in natural product research [16–19], and consequently, the problem of how to rapidly identify new NPs from complex mixtures has become a thorny challenge that needs to be resolved [18...