considering the effects of the individual gene mutations, and can reveal functional relationships between genes and pathways51,52. Mapping GI profiles has thus become a powerful approach for deciphering gene function. To systematically identify GIs for NatC, we performed...
Kubeczko, MarcinPolakiewicz-Gilowska, Annawiderska, KatarzynaLeniak, AleksandraMianowska-Malec, Martaanoszka, BarbarbaChomik, KonstantyGrandys, BarbaraLisovska, NatalyaBobek-Billewicz, BarbaraFrontiers in Oncology
Samples were mounted by applying 4-5 drops of ibidi mounting medium with DAPI (ibidi GmbH) to each well. Cells were examined using an Andor Dragonfly 500 confocal microscopy (Oxford instruments) equipped with an iXon 888 Life EMCCD camera and a 100×/1.49 oil-immersion objective. Images were...
baylyi (vipA-sfGFP) by applying a higher flow rate to the top flow channel (top channel 0.001μL/s, bottom 0.003μL/s for 30 s), thereby forcing aggressor cells into the tops of the observation channels. Next, E. coli (Ptac-mRuby3) cells were loaded from the bottom flow channel ...
Metacells were annotated as Monocytes /Macrophages or others, by applying a straightforward analysis of known cell type marker genes (e.g., Ear2, Fn1, Ccr2, Mrc1, Cd3d, Cd79b, and more). Subsets of Monocytes and Macrophages were obtained by hierarchical clustering of the confusion matrix ...
Next, we explored spatial transcriptome changes after striatal whole-blood injection applying the 10 × Genomics workflow while focusing on the brain regions comprising the perilesional area (striatum) and the CP. This approach allowed the extraction of gene expression data from these specific bra...
Quantification of individual metabolites was obtained from peak areas applying the correction factors determined by experiments at the equilibrium of magnetization (90o pulses, 30.00 s interpulse delay). Metabolite quantification was expressed as metabolite percentage relative to total metabolites. All data...