We identify male-specific impairments in long-term contextual memory and in synaptic gene expression, linked to altered miR-137 levels. We show that ATRX directly binds to the miR-137 locus and that the enrichment of the suppressive histone mark H3K27me3 is significantly reduced upon the loss...
A combination of single strand conformation polymorphism analysis (SSCP) and direct sequencing of RMST and ZNF804B were performed in mutation screening. The 46 primer sets were designed to cover all exons and flanking intronic regions of two predicted mRNAs. The size of amplicon is adjusted to ...
Whole genome sequencing allows to interrogate the non-coding portions of the genome and offers the possibility to provide a molecular diagnosis to patients with unsolved genetic disorders1. The challenge resides in the translation of the identified non-coding genetic variants to phenotype expression, si...
In each instance, what kind of chromosomal abnormality would you be looking for? a. A couple whose first child has Down syndrome b. A couple who have had three miscarriages in succession c. A pregnant 43-year-old woman and her 40-year-old husband d. A couple with two children wit...
Can I Exclude A Single Folder Using Copy-Item? Can I get AD User Office location? Can not execute powershell script from shared folder Can PowerShell be used to delete hidden USB/COM Ports? Can PowerShell restore previous versions of files/folders via Volume Shadow Services (VSS)? Can some...
A number of single nucleotide polymorphisms can associate with the risk of HNC reported in recent meta-analyses (6–10). Glutathione S-transferase theta 1 (GSTT1) gene produces an enzyme that plays a key role in the neutralization of electrophilic compounds. These compounds include carcinogens, ...
As a consequence, ATR-KD expressing cells display genomic instability at regions with accumulation of single-stranded DNA, like X–Y bodies, telomeres and ribosomal DNA, consistent with the essential role of ATR in replication, but not at DSBs. Differently from ATM- or DNA-PK...
22q11.2 deletion syndrome (22q11DS), a copy number variation (CNV) disorder, occurs in approximately 1:4000 live births due to a heterozygous microdeletion at position 11.2 (proximal) on the q arm of human chromosome 22 (hChr22) (McDonald-McGinn and Sull
of a protein. Using MISER, we generate a deletion landscape for the CRISPR protein Cas9. We find that the catalytically-deadStreptococcus pyogenesCas9 can tolerate large single deletions in the REC2, REC3, HNH, and RuvC domains, while still functioning in vitro and in vivo, and that these ...
(Supplementary Fig.1a–c). Although requiring several weeks to manifest, a striking phenotype ofHdac3IKOmice was reduced weight gain, which was evident in both males and females. In mice maintained on a standard diet, the body weight difference was first evident at 11 weeks, and became ...