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We identify male-specific impairments in long-term contextual memory and in synaptic gene expression, linked to altered miR-137 levels. We show that ATRX directly binds to the miR-137 locus and that the enrichment of the suppressive histone mark H3K27me3 is significantly reduced upon the loss...
Arrayed CRISPR libraries extend the scope of gene-perturbation screens to non-selectable cell phenotypes. However, library generation requires assembling thousands of vectors expressing single-guide RNAs (sgRNAs). Here, by leveraging massively parallel plasmid-cloning methodology, we show that arrayed libr...
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t.Errorf("Search failed: found value in an empty tree") } if btree.Delete(10) { t.Errorf("Delete failed: deleted value from an empty tree") } // Insert a single element. btree.Insert(10) // Search for the single element. if !btree.Search(10) { t.Errorf("Search failed: 10 ...
in response to calcium influx in neurons [7–11]. The inactivation of SRF leads to a decrease in the expression of such plasticity-linked genes asFos,Arc, andEgr1and produces a deficiency in hippocampal synaptic plasticity and learning [12,13]. The activity of SRF is also important during ...
of a protein. Using MISER, we generate a deletion landscape for the CRISPR protein Cas9. We find that the catalytically-deadStreptococcus pyogenesCas9 can tolerate large single deletions in the REC2, REC3, HNH, and RuvC domains, while still functioning in vitro and in vivo, and that these ...
In each instance, what kind of chromosomal abnormality would you be looking for? a. A couple whose first child has Down syndrome b. A couple who have had three miscarriages in succession c. A pregnant 43-year-old woman and her 40-year-old husband d. A couple with two children with...
A combination of single strand conformation polymorphism analysis (SSCP) and direct sequencing of RMST and ZNF804B were performed in mutation screening. The 46 primer sets were designed to cover all exons and flanking intronic regions of two predicted mRNAs. The size of amplicon is adjusted to ...
While 3D chromatin organization in topologically associating domains (TADs) and loops mediating regulatory element-promoter interactions is crucial for tissue-specific gene regulation, the extent of their involvement in human Mendelian disease is largely