We then reconstituted Rag expression in the qKO cells by stably re-expressing one Rag dimer at a time, thus generating the RagA/C, RagA/D, RagB/C and RagB/D cell lines, or a luciferase (Luc)-expressing line as a negative control. To assess the qualitative differences between the four ...
A GO term was significantly enriched if p-value ≤ 0.05 and FDR ≤ 0.05. Genes could be comprised in more than one term depending on the function of the encoded protein. The graph shows the most relevant GO terms. The GO term name is reported on the y-axis; the number of ...
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Identifying cargo effectors, especially orphan ones, remains one of the major challenges in the T6SS field. Here, we described an N-terminal T6SS delivery domain, PIX, that defines a class of polymorphic toxins and can thus serve as a marker for effectors. We used PIX to identify hundreds ...
One notable exception to this was reproducible EGFP expression on the ventral side of lobule IX in the most posterior region (Figure S1A), a site commonly associated with vestibular input, that we find no description of in any historical SC anterograde tracer literature. However, the lack of ...
Within each field or in vitro sample, gene expression was separately standardized to z-scores. Next, for each cluster’s set of genes within each dataset (in vivo or in vitro), these z-scores were averaged per sample. Finally, for each cluster, a one-sided t-test was performed comparing...
One can also see that the left area is intersected by a label and the border of the white 'zoom box' that indicates the section that is enlarged at the bottom of Fig. S4b. Text and white border are obviously absent in the right area. In the fist animation of#9one can see that the...
We used the HeLa nonpituitary cell line and our previously optimized Pit-1 protein-expression system to test the transcription potency of each mutant construct. The HA-tagged wild-type and mutant Pit-1s were introduced into HeLa nonpituitary cells with a rPRL promoter-driven luciferase reporter,...
(E) One representative P4 Isl1-Cre; PTXa IHC pair coimmunostained for GPSM2 and GNAI3, where the apparently normal right IHC served as an internal control (see text and STAR Methods). Top: GNAI3 and GPSM2 were planar polarized at the bare zone (arrow) in the control IHC but delocali...