The lung contains numerous specialized cell types with distinct roles in tissue function and integrity. To clarify the origins and mechanisms generating cell heterogeneity, we created a comprehensive topographic
In the violin plot, the red dots represent the median, the limits of the thick line correspond to quartiles 1 and 3 (25% and 75% of the distribution) and the limits of the thin line are 1.5× the interquartile range. e, Benchmarking of the method, using as a starting signal genes...
After quality control filtering, we retain 143,013 cells, evenly distributed between the conditions (Figures S3D and S3E). We identify 202 neuron types (Figures 2B and S4A–S4D), providing a high-resolution platform to study the neuron-type-specific impact of pathogenic proteins. Download: ...
33]. Within this context, psychological resilience emerges as a crucial factor in an individual’s ability to adapt to and recover from stress, adversity, or challenges [34]. Significantly, resilience has been linked to successful aging, possessing an impact that rivals the ...
A, anti-CTCF-immunoprecipitated samples were blotted with anti-BRG1 and anti-DDX5 antibodies. Anti-CTCF was used as positive control. Input, 2% of the cell lysate used for immunoprecipitation. B, anti-BRG1-immunoprecipitated samples were blotted with anti-CTCF and anti-DDX5 antibodies. Anti-BRG1...
(Suppl. Figure4A-B). Among all these markers, Sox2 showed a decreased level in AD-iPSCs compared with control cells (WB optical density: C-iPSC 1 ± 0.02, AD-iPSC 0.54 ± 0.07,P < 0.01 t test,n = 3; Suppl. Figure4B). In line with our previous data on AD-...
We demonstrated that multi-layer alterations of RNA modification “writer” are associated with patient survival and TME cell-infiltrating characteristics. We identified two distinct RNA modification patterns, characterized by a high and a low WM_Score. The WM_Score-high group was associated with wors...
We corrected the p value by Benjamini- Hochberg method for false discovery rate (FDR) control. Only probe sets differences that passed the test at a FDR ≤ 0.05 were considered significant. Fold-change (FC) was calculated to determine the magnitude of the difference. Probe sets were ...
a, Plot indicating neuronal and glial clusters that have lobule enrichment patterns that are significantly different from the cell type population as a whole (Pearson’s chi-squared, FDR < 0.001 indicated by dashed line).x-axis shows −log10-transformedqvalues;yaxis shows log2-transformed maximu...
(eBioscience, San Diego), and anti-I-A/I-E (BioLegend, Tokyo); PE-conjugated anti-IL-17A (eBioscience, San Diego), control IgG2a (eBioscience, San Diego), and anti-I-A/I-E (BioLegend, Tokyo); PE-Cy5-conjugated anti-CD4 (BioLegend, Tokyo); PE-Cy7-conjugated anti-CD4 (Bio...