The aim of this study was to analyse the range of emerging mutations in herpesvirus by whole genome deep sequencing. We tested human herpesvirus 6 treatment with novel antiviral K21, where evidence indicated distinct effects on virus envelope proteins. We treated BACmid cloned virus in order to ...
Whole genome sequencing (WGS), variant calling and genes to test DNA was obtained from cell line pellets with the Qiagen Quick-Start DNeasy Blood and Tissue Kit (cat. no. 69506). DNA samples were submitted to the Genomics Platform at the Broad Institute of MIT and Harvard. Whole genome se...
QKI-RAF1 and FYCO-RAF1 have been identified as rare alterations in PAs using whole-genome sequencing,6 whereas SRGAP3-RAF1 was first reported as a tandem duplication event.7,8 Recently, ATG7-RAF1 fusions were reported in anaplastic Pleomorphic Xanthoastrocytomas without BRAF-V600E.9 Although...
Whole-exome sequencing (WES) and transcriptome sequencing (RNA-seq) were conducted to obtain the mutation burden and genomic expression pattern. Results We found unique profiles of the intestinal microbiome, metabolome, and host genome between RCC and LCC. The bacteria Flavonifractor plautii (Fp) ...
4 Comprehensive whole-genome sequencing studies have discovered a diversity of novel RAF-fusion gene combinations. In particular, multiple gene fusions harboring CRAF (or RAF1), a serine/threonine kinase first discovered as the v-raf oncogene in transforming mouse sarcoma virus,5 have been reported...
Proportion of genes in COGs sorted from each whole genome compared to the proportion of genes in COGs sorted from the essential genes for each genome. For all strains used in the study, the genes of the genome were sorted into COGs and the number of genes in each COG is expressed as a...
These mutants showed constitu- tive activity and Y705 phosphorylation in the absence of cytokine signaling.11 Similar results have been obtained in large granular lymphocytic leukemia using whole genome sequencing. In this disease, 40% of patients express a mutated form of...
Whole genome sequencing reveals complex resistome features of Klebsiella pneumoniae isolated from patients at major hospitals in Trinidad, West Indies Antibiotic-resistantKlebsiella pneumoniaeis a human pathogen of major global concern due to its ability to cause multiple severe diseases that are often di...
Citation11 Similar results have been obtained in large granular lymphocytic leukemia using whole genome sequencing. In this disease, 40% of patients express a mutated form of STAT3 on its SH2 domain, and this mutation induces a constitutive phosphorylation of the transcription factor.Citation12 ...
Whole-genome and whole-exome sequencing of 47 hindbrain ependymomas reveals an extremely low mutation rate, and zero significant recurrent somatic single nucleotide variants. Although devoid of recurrent single nucleotide variants and focal copy number aberrations, poor-prognosis hindbrain ependymomas ...