The aim of this study was to analyse the range of emerging mutations in herpesvirus by whole genome deep sequencing. We tested human herpesvirus 6 treatment with novel antiviral K21, where evidence indicated distinct effects on virus envelope proteins. We treated BACmid cloned virus in order to ...
Whole genome sequencing (WGS), variant calling and genes to test DNA was obtained from cell line pellets with the Qiagen Quick-Start DNeasy Blood and Tissue Kit (cat. no. 69506). DNA samples were submitted to the Genomics Platform at the Broad Institute of MIT and Harvard. Whole genome se...
CNS cancer: TERT alterations define high-risk neuroblastoma.Only a few genes are recurrently mutated in patients with neuroblastoma. In a new study, whole-genome sequencing was used to analyse tumour samples from 39 patients with high-risk neuroblastoma and 17 patients with low-risk neuroblastoma....
These mutants showed constitu- tive activity and Y705 phosphorylation in the absence of cytokine signaling.11 Similar results have been obtained in large granular lymphocytic leukemia using whole genome sequencing. In this disease, 40% of patients express a mutated form o...
Whole genome sequencing reveals complex resistome features of Klebsiella pneumoniae isolated from patients at major hospitals in Trinidad, West Indies Antibiotic-resistantKlebsiella pneumoniaeis a human pathogen of major global concern due to its ability to cause multiple severe diseases that are often di...
Heyn H, Vidal E, Sayols S, Sanchez-Mut JV, Moran S, Medina I, Sandoval J, Simo-Riudalbas L, Szczesna K, Huertas D, Gatto S, Matarazzo MR, Dopazo J, Esteller M: Whole-genome bisulfite DNA sequencing of a DNMT3B mutant patient. Epigenetics. 2012, 7: 542-550. 10.4161/epi.20523...
Transcriptome sequencing has identified multiple subtypes of B-progenitor acute lymphoblastic leukemia (B-ALL) of prognostic significance, but a minority of cases lack a known genetic driver. Here, we used integrated whole-genome (WGS) and -transcriptome sequencing (RNA-seq), enhancer mapping, and ...
In this study, we sought to describe its unique histopathologic features and to identify the genetic alterations that underpin SPCRP using massively parallel whole-exome and targeted sequencing. The morphologic and immunohistochemical features of SPCRP support the invasive nature of this subtype. Ten ...
Whole cell extracts were prepared using RIPA lysis buffer (10 mM Tris HCl, pH 8.0, 1mM EDTA, 150 mM NaCl, 1% Triton-X-100, 0.1% sodium deoxycholate, 0.1% SDS) in the presence of 1X Halt protease inhibitor (Thermo Fisher) and 1 mM PMSF (Sigma, St Louis, MA). The resulting protein...
Whole-genome sequencing identifies genetic alterations in pediatric low-grade gliomas. Nat. Genet. 45, 602–612 (2013). Article CAS Google Scholar Wülling, M. et al. The nature of giant cell tumor of bone. J. Cancer Res. Clin. Oncol. 127, 467–474 (2001). Article Google Scholar ...