The advent of genetically engineered systems, including transgenic animals and recombinant viral vectors, has facilitated a more detailed understanding of the molecular and cellular substrates regulating brain function. In this review we highlight some of the most recent molecular biology and genetic ...
Define SimBiology model components’ roles PKModelMap objectwill be removed in a future release. Use a combination ofEstimatedInfo object,CovariateModelobject, cell array of character vectors, andsbiodose. Seesbiofitandsbiofitmixedfor illustrated examples. ...
Among the three clusters of tumor cells, those in C2 were generally more differentiated than the others (Figs.2C,S4A). Note that the cell clusters defined by gene expression profiles did not show such significant differences in their differentiation stages (Fig.S4B). Furthermore, compared to th...
To characterize two newly identified naturally occurring mutants, we used site-directed mutagenesis to construct expression vectors containing these two point mutations. After transfection in HEK293 cells, both wild-type (GPR103-WT) and the R371W mutant (GPR103-R371W) mediated a significant inhibit...
1× 106attP-Hygro-TK-attB ES cells were electroporated with 5 μg of the integrase vectors and 200 ng of a control plasmid containing a functional neomycin cassette to control for transfection efficiency. Electroporation was performed using the Neon transfection system (Invitrogen) (3 × 140...
The type II secretion system (T2SS), a virulence pathway in many pathogens, belongs to this superfamily. A knowledge gap in understanding of the T2SS is the molecular role of a small “pseudopilin” protein. Using multiple biophysical techniques, we have deciphered how this missing component ...
a Immunoblot analysis of EMT-TFs, EMT markers and S100 proteins in a panel of PDAC cell lines. b Analysis of the transcription of ZEB1-regulated S100 genes in epithelial PDAC cells. BxPC-3 and SU.86.86 cell lines were transfected with the plasmid vectors expressing GFP-tagged ZEB1 or GFP ...
Knowledge of the flight behavior of local vectors is of paramount importance in mosquito control programs. The following study defined the recapture rate of wild-caught, unengorged Anopheles darlingi females at 0, 400, and 800 m from a fixed release point in Belize, Central America, using a ...
The Expression property is a character vector or cell array of character vectors, where each character vector represents the relationship between a parameter and one or more covariates.
Recent efforts have refined the approach using pools of purified protein fragments for in vivo immunization combined with ELISA based screening and recombinant DNA methods to rapidly clone the heavy and light chains into mammalian expression vectors to produce multiple high quality antibodies from a ...