including expression of some basal epithelial cell markers44,45. Gene expression profiling fromBRCA1heterozygous breast tissue showed similarities between luminal progenitor cells and basal-like breast tumors, and between luminal mature cells and luminal A/B tumors44....
Intestinal stromal cells are known to modulate the propagation and differentiation of intestinal stem cells1,2. However, the precise cellular and molecular mechanisms by which this diverse stromal cell population maintains tissue homeostasis and repair are poorly understood. Here we describe a subset of...
Analysis of plants expressing functional GFP-tagged versions of GL1, GL3 and TRY show that the proteins are localized in trichome nuclei. These results have been used to model trichome initiation in terms of protein interactions and threshold levels of activator complex. 展开 ...
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These cells have a large in-frame deletion that removes 67% of the coding sequence of the Wiz gene (including zinc fingers 2–5), likely resulting in a null allele. Western blot analysis confirmed that an epitope within the deleted region is not detected in Wizdel cells. Wizdel cells may...
We find that cooperative repression by tetrameric Lac repressor increases with both quality and proximity of the interacting operators. A short distance of 92 bp allows effective repression by two very weak operators (O3, O3). The cooperativity of lac operators is discussed in terms of a local ...
Genome-wide modes of gene regulation by FLC and SVP were identified and classified into four representative clusters in leaves and apices (clusters A to D in leaves and A’ to D’ in apices in Figure1D,E; Additional files5and8). The clusters are based on the contribution of the terms of...
We next tested directly whether tumor growth was dependent on both p53 and MYCN by generating GTML mice deficient in functional p53, using a mouse model in which the endogenous Trp53 gene is replaced with a knockin allele (Trp53KI) encoding a 4-hydroxytamoxifen (4-OHT)-regulatable p53ERTAM...
1. Yeast strains and plasmids are described in Tables 1 and 2, respectively.All yeast strains are of the SK1 background (25). All plasmids are derived frompMJ113 or pMJ115, which contain pBR322 sequences, the URA3 gene, andarg4-nsp or arg4-bgl alleles, respectively (67). PCR ...
RNA splicing shapes the gene regulatory programs that underlie various physiological and disease processes. Here, we present the SCASL (single-cell clustering based on alternative splicing landscapes) method for interrogating the heterogeneity of RNA splicing with single-cell RNA-seq data. SCASL resolve...