Instead, it is a trajectory of cell states regulated by distinct factors essential for metastatic progression. Results Temporal profiling of gene regulation during osteosarcoma metastasis We performed ATAC-seq and RNA-seq on the metastatic human osteosarcoma cell line MG63.3-GFP grown in vitro, as ...
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seq datasets were aligned with CCA25,35, and the scATAC cells were labeled based on the nearest snRNA-seq cells, which agreed with the manual annotations for 65% of cells, with mislabeled cells typically being labeled as the nearest cell type in the differentiation trajectory (Extended Data ...
seq datasets were aligned with CCA25,35, and the scATAC cells were labeled based on the nearest snRNA-seq cells, which agreed with the manual annotations for 65% of cells, with mislabeled cells typically being labeled as the nearest cell type in the differentiation trajectory (Extended Data ...
In crossing situation:(2) In over-taking situation, the equation is:(3) Where: Sources of range error in Global Positioning System constant signal arrival C/A: ±3m, Signal arrival P(Y): ±0.3m, Ionospheric effects:± 5m, Ephemeris errors:± 2.5m, Satellite clock errors: ± 2m, Multipa...
Given these results, detailed mapping of the developmental path or paths that progenitor cells take during differentiation to their end state or fate both in vivo as well as in PSC-derived systems has now become a primary objective of the field. Similar challenges in obtaining pure cell ...
In a good system, most people are good. In an evil system, most people are evil. The wavering majority changes the game through their participation or their absence. In the example of the crowd above during the final judgment of Jesus’ fate, it is the angry crowd who chooses to let a...
We plotted intermolecular hydrogen bonds between α and β tubulin chains, using trajectory analysis across a 200-ns time course (Fig. 4). Mutation W407X was predicted to reduce H-bonding throughout the simulation, trending toward zero H bonds over the final 80 ns (Fig. 4D), whereas ...
46. The chromatin landscape at single-cell resolution is uniquely suited to reconstruct the lineage between progenitor populations and malignant cells in a tumor. To determine whether tumor subtypes were associated with distinct cells of origin, we performed Monocle trajectory analysis on snATAC-seq ...
First, residues ranges for optimization were chosen by simple manual inspection of where along α1 slight deviations in its trajectory would be likely to bring it into closer alignment with the PulG reference helix for that subunit. Secondly, for each of these n residues, one at a time ...