(US Biologicals), 0.1 mM β-mercaptoethanol (Invitrogen), 1% non-essential amino acids (Hyclone), 1% N2 and 2% B27 supplements (Invitrogen), 20 ng/ml basic fibroblast growth factor (Invitrogen), epidermal growth factor (PeproTech), platelet-derived growth factor (PeproTech), and ...
For base editing screens, we used the control gRNAs (NT, intergenic and nonessential) to generate a control distribution and implemented MAGeCK (RRA) to compare against the test gRNAs. For prime editing screens, we used the same approach but the control distribution was generated from pegRNAs ...
essential amino acids and essential fatty acids, as well as all vitamins and minerals from the group of micronutrients, must be consumed in appropriate quantities A diet high in processed and refined foods can lead to excessive intake as well as deficiencies of certain nutrients Non-essential ...
In the present paper, by exchanging the corresponding regions between Nlalpha1 and Nlalpha3 to generate different chimeras, amino acid residues or residue clusters in the regions outside the six loops were found to play essential roles in agonist binding, especially for the amino acid clusters ...
INTRODUCTIONSialic acids are essential components of glycoconjugates on deuterostomia cells [1]. They have been shown to be involved in many biological pro... A Blume,W Weidemann,U Stelzl†,... 被引量: 0发表: 0年 加载更多来源期刊 American Journal of Human Genetics 1999 Jun; 研究点推荐...
Non-significant changes are white, significant values are depicted in a gradient of red. Amino acids with no data are in gray. Site-specific cysteine incorporation in XcpJp provides XcpHp:Jp interaction sites Cysteine cross-linking The knowledge that a significant structural interaction interface for...
The last amino acid at the C terminus of the GFP minimal domain is a neutral Ile residue. Thus, ionic charge interaction at this position is probably not an essential factor in determining the GFP structure and function. Since Ile is a hydrophobic amino acid, it is possible that the hydroph...
Similarly, in retinal cells, studies of Brn3b null mice indicate that Brn3b is essential for controlling genes whose products are required for the organization of the cytoskeleton, normal cell polarity and axon outgrowth. Therefore, Brn3b is not required for the initial commitment of retinal ...
DIABETIC CAPD PATIENTSKyu-Hyang Cho, Jun-Young Do, Soek-Hui Kang, Jong-Won Park, Kyung-Woo YoonDepartment of Internal Medicine, Yeungnam University Hospital, Daegu, Korea.Purpose: Preservation of residual renal function (RRF) in peritonealdialysis patient is essential to improve clinical outcomes. ...
The ability of tubulin monomers to self-assemble into large polymers in the presence of GTP, and to disassemble upon GTP hydrolysis, is an essential characteristic of microtubule dynamics, and is critical to many biological processes such as cell motility, mitotic chromosome segregation, and ...