To chart cell composition and cell state changes that occur during the transformation of healthy colon to precancerous adenomas to colorectal cancer (CRC), we generated single-cell chromatin accessibility profiles and single-cell transcriptomes from 1,00
Fig. 1: Single-cell atlas of expression and chromatin accessibility in CRC development. a, Summary of the samples in this study. The bar chart shows the number of normal/unaffected colon tissues (gray), adenomas (purple) and CRCs (red) assayed for each patient. Locations of samples assayed...
et al. Identification and prospective isolation of a mesothelial precursor lineage giving rise to smooth muscle cells and fibroblasts for mammalian internal organs, and their vasculature. Nat. Cell Biol. 14, 1251–1260 (2012). Article CAS PubMed PubMed Central Google Scholar Guo, Z. et al. ...
et al. Biochemical isolation of myonuclei employed to define changes to the myonuclear proteome that occur with aging. Aging Cell 16, 738-749 (2017).Cutler,A.A., Dammer,E.B., Doung,D.M., Seyfried,N.T., Corbett,A.H. and Pavlath,G.K. (2017) Biochemical isolation of myonuclei ...
Indeed, species specialization is an overall culmination of the function of all the cell types within that species; thus, they may follow similar evolutionary principles. There have been several ways proposed to classify species. One is based on the notion of reproductive isolation. However, this ...
Immune checkpoint inhibitors have transformed the treatment landscape of non-small cell lung cancer (NSCLC). However, accurately identifying patients who will benefit from immunotherapy remains a challenge. This study aimed to discover potential biomarke
Notably, retroviral scanning represents an instrumental technique for the retrospective identification of rare populations (e.g. somatic stem cells) that lack robust markers for prospective isolation. 展开 关键词: Genetics, Issue 123, Moloney leukemia virus, Integration sites, Virus-host interaction, ...
define Description Syntax Examples Links Description The define of Miscellaneous for PHP defines a named constant. Syntax define( string $constant_name, mixed $value ): bool Parameters constant_name The name of the constant. NOTE: It is possible to define() constants with reserved or even invalid...
Isolation of retinal progenitor cells from post-mortem human tissue and comparison with autologous brain progenitors expressed doublecortin, beta-III tubulin, and glial fibrillary acidic protein, which is consistent with increased lineage restriction in subsets of cultured ... H Klassen,B Ziaeian,II Ki...
Cell isolation and culture Primary mouse stem Leydig cells isolation was performed according to a previous article15. The testes were dissected from Nes-GFP or C57BL/6 mice, and then we removed the tunica albuginea carefully from each testis and minced the testes into small pieces. The interst...