Apoptosis is a fundamental process for the elimination of damaged or unwanted cells, and is a key aspect of development. It is triggered by pro-apoptotic genes responding to the intrinsic pathway that senses cell stress or the extrinsic pathway that responds to signals from other cells. The ...
suggesting coordination of these processes. We studied fetal germ-cell fates and discovered that both apoptosis and differentiation initiate in clonally related clusters. Lineage tracing confirmed that germ cells die as clones independent of intercellular bridges, suggesting that shared intrinsic properties ...
Defects in the prelamin A processing enzyme caused by loss-of-function mutations in the ZMPSTE24 gene are responsible for a spectrum of progeroid disorders characterized by the accumulation of farnesylated prelamin A. Here we report that defective prelam
A number of studies have suggested a role for estrogen in the regulation of DNA repair activity. Furthermore, mutations or epigenetic silencing in DNA repair genes have been associated with the sensitivity of cancers to hormonal therapy. The molecular basis for the progression of cancers from ...
To distinguish between decreased intrinsic catalytic ability versus the failure of the mutant protein to be recognized as an autosubstrate, equivalent amounts of cleaved wild-type and mutant GST-CASP10 were assayed using a DEVD peptide substrate (Figure 4C). We observed significantly reduced ...
Here, we report that while different types of DNA damage are efficiently repaired in stimulated T cells, they result in massive apoptosis of resting T cells. Mechanistically, DNA damage in resting T cells activates the ATM/ATR/DNA-PKcs signaling pathway but fails to induce the formation of 纬...
Spliceosome mutations are frequently found in myelodysplasia. Splicing alterations induced by these mutations, their precise targets, and the effect at the transcript level have not been fully elucidated. Here we report transcriptomic analyses of 265 bon
apoptosis, control protein translation and export peptides or mitochondrial DNA (mtDNA)41. These functions may require the interplay between mitochondria and other organelles, such as the endoplasmic reticulum, peroxisomes, and lysosomes42. Hence, mitochondria can be considered a central hub for the ...
Thus, deficient DNA duplication is intrinsic to myotubes and not due to the reactivating conditions.11 We have argued that defective DNA duplication and DNA damage occur in most terminally differentiated cell types upon reactivation.11 In this view, terminal differentiation entails critical, ...
While the activated p53 promotes cell cycle arrest and DNA repair, it also drives the intrinsic and extrinsic apoptosis pathway by the upregulation of pro-apoptosis factors3. Since p53 was not fully activated in resting CD4+ T cells (Fig. 3g), we speculated that p53-independent pathways might...