深度测序的第一步是构建文库,文库是测序样本的DNA或RNA序列的集合。文库构建的关键是将DNA或RNA样本转化为适合测序的文库,其中包括以下几个主要步骤: (1)样本处理:首先需要对DNA或RNA样本进行预处理,如DNA提取、RNA提取、PCR扩增等。 (2)文库构建:将经过处理的DNA或RNA样本转化为测序文库,一般采用不同的构建方法,...
Trancriptome Analysis of Testis and Liver for Androstenone by Using RNA Sequencing Boar taint is an unpleasant odor and flavour of the meat. Boar taint occurs in a high proportion of uncastrated male pigs due to high level of accumulation of androstenone and skatole in the adipose tissue. It...
Low-quality read pairs of single-cell RNA sequencing (scRNA-seq) data were filtered out if at least one end of the read pair met one of the following criteria: (1) ‘N’ bases account for ≥10% of the read length; (2) bases with quality <5 account for ≥50% of the read length;...
RNA deep sequencing of the Atlantic cod transcriptome. Comp Biochem Physiol Part D Genomics Proteomics 2011;6:18-22.Johansen SD, Karlsen BO, Furmanek T, Andreassen M, Jorgensen TE, Bizuayehu TT, Breines R, Emblem A, Kettunen P, Luukko K, Edvardsen RB, Nordeide JT, Coucheron DH, Moum ...
The application of deep-sequencing technologies to transcriptome analysis has recently been attracting a great deal of attention. The determination of the exact boundaries and relative levels of the whole set of RNA molecules transcribed from a genome improves our understanding of functional elements, fa...
Using next-generation sequencing, the unbiased and absolute quantification of the small RNA transcriptome is now feasible. Neuroblastoma(NB) is an embryonal tumor with highly variable clinical course. We analyzed the small RNA transcriptomes of five favorable and five unfavorable NBs using SOLiD next-...
这篇论文研究的是Single-cell RNA sequencing (scRNA-seq) denoising, 也就是单细胞RNA测序的降噪,由于数据扩增和数据丢失等问题,会干扰scRNA-seq的数据分析,因此需要有降噪技术用于稀疏的scRNA-seq数据,作者提出了一种deep count autoencoder network (DCA),通过negative binomial noise model with or without zero-...
coli /itsRNAs using a deep sequencing approach has shown that many as-yet-undiscovered sRNAs are potentially encoded in the itE. coli /itgenome. We constructed the itEscherichia coli /itSmall RNA Browser (ECSBrowser; urlhttp://rna.iab.keio.ac.jp//url), which integrates the data for ...
Validated putative miRNAs. (A) Sequences for both mature miRNA and predicted pre-miRNA. Copy number refers to the occurrences of the mature sequences in the E15.5 whole brain small RNA sequencing analysis. (B) RNAfold prediction of the stemloop hairpin structure. The colours in the vertical ...
Biogenesis and molecular function are two key subjects in the field of microRNA (miRNA) research. Deep sequencing has become the principal technique in cataloging of miRNA repertoire and generating expression profiles in an unbiased manner. Here, we describe the miRGator v3.0 update (http://mirgato...