Cell activity in ECM-derived hydrogels: (A) Cell viability assessment (green = live, magenta = dead, scale bar = 200 µm); (B) macroscopic images of cell-laden hydrogels over time in culture; (C) quantification of hydrogel area over time; (D) quantification of methylene...
The inverted method was superior to the vented method, as shown by a higher coronary perfusion efficiency, more cell debris outflow, higher collagen and elastin content inside the aortic valve, lower DNA content, and better retention of the heart shape after decellularization. To our knowledge,...
As we are mainly interested in musculoskeletal tissue engineering, we used the murine C2C12-cell line. Here, none of the washing solutions showed toxicity in the cell assay. Figure 4 shows the results of the harshest protocol employing decellularization with 1% SDS for 120 min (Fig. 4). ...
Physical methods of decellularization such as freeze thaw can help disrupt cell membranes19 as well as induce apoptosis of chondrocytes20. However the effect on degradation of the extracellular matrix structure has been proven to be insignificant. Szarko et al. reported no differences in ...
2 show the H&E staining of the specimens. As it is shown in Fig. 2 B2–B3 and D2-D3, the non-channeled specimens of both the PBB and the shaker had cell nuclei sporadically (indicated with red arrows). On the other hand, cell nuclei were removed completely from the depth of the...
We previously described the production of vascularized and cell-compatible scaffolds, from porcine ear grafts. In this study, we --- applied findings directly to human auricles harvested from postmortem donors, providing a perfusable matrix that retains the ear’s original complexity and hosts new ...
However, small number of BMSCs and loss of stem cell characteristics after expansion in vitro limited clinical use of BMSCs. In the present study, osteoblasts were cultured to lay down extracellular matrix (ECM) and then the cells were removed (decellularization) to generate ECM coating ...
Cell proliferation was determined using WST-1 (Roche©), as mentioned previously. As assay control, a mixture of growth medium and WST-1 without cells was used. Cell length and circularity were measured using ImageJ 1.49 software from the National Institutes of Health, USA. Measurements were ...
are involved in the development of immune responses12,13; (iii) some lipids hamper recellularization by impairing cell attachment14; and iv) certain lipids may act as a source of pro-inflammatory fatty acids, such as arachidonic acid15. Moreover, residual antigens (dsDNA) in the bio-ECM ...
The extracellular matrix (ECM) of the tubes provides not only structural support to the cells, but also is able to self-modulate due to cyclical hormonal stimulation in the female reproductive tract [14,15]. The tubal ECM molecular signaling is able to stimulate cell proliferation in the mucosa...