TEFM expression was analyzed by quantitative real-time PCR, western blot, and immunohistochemistry analysis in HCC tissues and cell lines. The effects of TEFM on HCC cell growth and metastasis were determined by cell proliferation, colony formation, flow cytometric cell cycle and apoptosis, migration...
Bax was found to be decreased in Huh7 and Hep3B cells, and increased in SNU449, when SAG was overexpressed. Real-time PCR affirms that attenuated Noxa and SARM under SAG overexpression is due to a post-transcriptional event, suggesting the involvement of SAG-dependent UPS. Actin was used ...
To decide whether the oncogenic function of USP35 in HCC cells relies on ABHD17C, we overexpressed ABHD17C in USP35-deficient Hep3B and SNU449 cells and found that ABHD17C overexpression rescued the reduced levels of p-PI3K and p-AKT caused by USP35 knockdown (Fig.5A, B). In addition...
HCCLM3/mTOR cells as compared to controls (Fig.2F). Similar findings were also observed in HepG2/mTOR cells (Fig.S2B, C). By contrast, sorafenib increased intracellular ROS level in SNU886/shmTORcells (Fig.2G) and SNU398/shmTORcells (Fig.S2D). Additionally, knockdown of mTOR reduced t...
All mice experiments were approved by the Animal Care and Use Committees of Seoul National University or Hanyang University (certification numbers: SNU-140320-2-9 and HY-IACUC-18-0048). BM Transplantation BM cells from Flt3–/–, Zbtb46-DTR, BDCA2-DTR, or Pdl1–/– mice64 (housed in ...
The cell lines, DLD-1, Caco-2, SW480, HCT-116, HCT-15, RKO, LoVo, and CCD 841 CoN, were obtained from American Type Culture Collection (ATCC, USA); and KM12 were acquired from Korean Cell Line Bank (KCLB, SNU). The cells were grown in the recommended media supplemented with a ...
Many path- ogens can survive in host cells by modulating the host defense mechanism like the autophagy system8. However, the molecular mechanism behind the interaction between VvhA and the defense system for host cell death during V. vulnificus infection has not been clarified yet. Autophagy is...
RBE, QBC939, HUCCT1, CCLP-1, SNU-869 and HCCC-9810 cell lines were sourced from Procell (Wuhan, China) with STR identification reports and tested to exclude mycoplasma contamination. Cells were cultured in RPMI-1640 medium supplemented with 10% FBS (Servicebio, China) at 37 °C in 5%...
Derivation and characterization of new human embryonic stem cell lines: SNUhES1, SNUhES2, and SNUhES3. Stem Cells 2005; 23: 211–219. Article Google Scholar Tsuda H, Jafar-Nejad H, Patel AJ, Sun Y, Chen HK, Rose MF et al. The AXH domain of Ataxin-1 mediates neurodegeneration ...
Hepatocellular carcinoma (HCC) represents a global health challenge with limited therapeutic options. Anti-angiogenic immune checkpoint inhibitor-based combination therapy has been introduced for progressed HCC, but improves survival only in a subset of