triterpene saponins(皂苷)、 研究方法及结果:转录组分析了三个组织(叶、根、花),组装后有107,340 unigenes,9,908 unigenes were assigned to 135 KEGG pathways,270 unigenes were identified to be involved in triterpene saponin biosynthesis。 深度剖析:本文做了什么呢?没有处理,直接取了三种组织的样品,做转...
ARTICLE Received 15 May 2016 | Accepted 17 Mar 2017 | Published 4 May 2017 DOI: 10.1038/ncomms15324 OPEN Sequencing and de novo assembly of a near complete indica rice genome Huilong Du1,2,*, Ying Yu1,*, Yanfei Ma1,*, Qiang Gao1,*, Yinghao Cao1,*, Zhuo Chen1,2, Bin Ma1, Ming...
Zhu, 2014 De novo mutations discovered in 8 Mexican American families through whole genome sequencing. BMC Proc. 8: S24.Wang H, Zhu X. De novo mutations discovered in 8Mexican American families through whole genome sequencing. BMC Proc. 2014; 8 (suppl 2):S24....
De novo sequencing, assembly and analysis of the genome of the laboratory strain Saccharomyces cerevisiae CEN.PK113-7D, a model for modern industrial biotechnology Jurgen F Nijkamp, Marcel van den Broek, Erwin Datema, Stefan de Kok, Lizanne Bosman, Marijke A Luttik, Pascale Daran-Lapu...
In fact, the emerging picture of the "pan genome" (Morgante et al. 2007) suggests that even sequencing genomes within a species may benefit from a de novo approach rather than a resequencing approach. However, the challenge of de novo sequencing with larger genomes is that assembly becomes ...
Genome sequencing andde novoassembly Both Flye and Hifiasm produced assemblies with high contiguity (Table1, Fig.2). The Hifiasm assembly was more contiguous than that generated by Flye, with N50 of 22 Mb and 2.9 Mb and 324 and 764 contigs for Hifiasm and Flye, respectively. The size distri...
For indels a total of 11 novel, 15 loss of function and 19 de novo indels were assayed. variants and 54 putative somatic variants. Sanger sequencing validation shows a low FDR for SNVs (4.1%, 1/24), whereas de novo indel validation was made difficult by de novo indels mostly occurring ...
Here we used whole-genome sequencing (WGS) to identify both rare de novo and inherited genetic risk factors for ASD in both coding and non-coding regions of the genome in the largest cohort of multiplex families evaluated to date. Results We analyzed high-coverage WGS data from 2,308 ...
In general, high-quality resequencing, a stringent bioinformatic pipeline, IGV manual curation, and validation via Sanger sequencing ensured the accuracy of DNMs we detected. The evolutionary history of Sus scrofa revisited through the DNM rate We considered the results of PSMC and two other methods...
N. et al. Genomic patterns of de novo mutation in simplex autism. Cell 171, 710–722.e12 (2017). CAS PubMed PubMed Central Google Scholar C Yuen, R. K. et al. Whole genome sequencing resource identifies 18 new candidate genes for autism spectrum disorder. Nat. Neurosci. 20, 602–...