[1]State Key Laboratory of Plant Cell and Chromosome EngineeringShisong Ma[5]University of Chinese Academy of SciencesZhixi Tian中国科学:生命科学(英文版)Shen Y T, Liu J, Geng H Y, et al. De novo assembly of a Chi⁃ nese soybean genome[J]. Science China Life Sciences, 2018, 61(8)...
De novo assembly of soybean wild relatives for pan-genome analysis of diversity and agronomic traits 来自 掌桥科研 喜欢 0 阅读量: 747 摘要: Wild relatives of crops are an important source of genetic diversity for agriculture, but their gene repertoire remains largely unexplored. We report the ...
Complete information on transcriptomes can easily be generated via high-throughput mRNA sequencing technology, which is a powerful and cost-effective tool for gene expression profiling in non-model organisms without a reference genome10. Illumina transcriptomede novosequencing and assembly have been success...
psammophila to that of S. suchowensis was equal to 1.645, indicating that the genome size of S. psammophila is estimated to be approximately 699–706 Mb (Fig. S1, Table S1). Illumina sequencing, de novo assembly and functional annotation of unigenes. Equal quantities of RNA ...
Transcriptome sequencing and de novo assembly We conducted de novo assemblies of one leaf and 16 fruit transcriptomes from 13 wild and cultivated berry fruit species. These species belong to eight plant genera and seven families: Berberidaceae (B. buxifolia), Caprifoliaceae (L. caerulea), Elaeocar...
近日,中国科学院遗传与发育生物学研究所刘志勇研究员与赵玉胜研究员团队合作在Nature Communications上发表了题为“A membrane associated tandem kinase from wild emmer wheat confers broad-spectrum resistance to powdery mildew”的研究论文,图位克隆了野生二粒...
Using transcriptome sequencing, de novo assembly and DGE analysis, candidate genes encoding putative enzymes responsible for late steps in RIN and IRN synthesis were identified. Our results lay a foundation for construction of the RIN and IRN biosynthetic pathways that will in turn aid the study of...
De novotranscriptome assembly Reads were cleaned using Trimmomatic (version 0.39) [37] with default settings. This process involved trimming adapter sequences, removing reads containing poly-N regions and deleting reads with quality less than 3. Additionally, reads shorter than 36 bases were excluded...
Using the latest sequencing and optical mapping technologies, we have produced a high-qualityde novoassembly of the apple (Malus domesticaBorkh.) genome. Repeat sequences, which represented over half of the assembly, provided an unprecedented opportunity to investigate the uncharacterized regions of a...
Elucidation of the genomic organizations of transgene insertion sites is essential for the genetic studies of transgenic plants. Herein, we establish an analysis pipeline that identifies the transgene insertion sites as well as the presence of vector backbones, through de novo genome assembly with high...