Thus, this displacement assay was suitable for screening and binding affinity determination of ligands in the low micromolar KD range (Supplementary Fig. 2b). We evaluated the established set of ligands (1–13) against all LC3/GABARAPs. A representative data set for LC3B is shown in Fig. 2a...
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Namely, the C 3 (+) triskelion and the assembled capsids were strongly antimicrobial with no apparent haemolytic activities at micromolar concentrations. Minimum inhibitory concentrations (MICs) were typical of polypeptide antibiotics within the phenotypic tolerance of bacteria to antibiotics47, while the...
Micromolar concentrations of CALP1 together with CaM,2 but neither CALP1 nor CaM alone, were found to activate phosphodiesterase in the absence of Ca2+. This demonstrated that the combination of CALP1 and CaM functioned similarly to a combination of Ca2+ and CaM. These results implied but did...
Moreover, compound 1 was characterized for its affinity to the ligand binding domain of PPARγ by isothermal titration calorimetry (ITC), yielding a measured dissociation constant of KD= 0.8 ± 0.1 μM, and a molar ratio of one ligand per protein molecule (Fig. 5b). This KD...
consistent with either increasing or decreasing size as expected from the design model (Fig.2a,dand Extended Data Fig.4). Timed SEC-binding experiments further revealed that these rings transition in oligomeric state on the order of hours at low micromolar concentrations of peptide and protein (Su...
These studies demonstrated that, when normalized to the original cell volume and calculated on molar basis, FTY720 and FTY720-P dramatically accumulated several hundredfold within immune cells reaching micromolar concentrations. The amount and distribution of FTY720 was differentially affected by SphK1- ...
consistent with either increasing or decreasing size as expected from the design model (Fig.2a,dand Extended Data Fig.4). Timed SEC-binding experiments further revealed that these rings transition in oligomeric state on the order of hours at low micromolar concentrations of peptide and protein (Su...
μM micromolar 2-ME 2-mercaptoethanol (also called β-ME) ATCC American Type Culture Collection ATP adenosine 5′-triphosphate CDNA complimentary DNA cm2 square centimeters CMV cytomegalovirus CPAP centrosomal P4.1-associated protein CPE cytopathic effects DMEM Dulbecco′s modified Eagle′s medium DMSO...
The smaller the dissociation constant, the more tightly bound the peptide is; for example, a peptide with a nanomolar (nM) dissociation constant binds more tightly than a peptide with a micromolar (μM) dissociation constant. In one embodiment, the present PMMA-binding peptides have a KD ...