Protein and biotin were mixed in a 1:1 molar ratio in PBS on ice for 2 hours. The reaction mixture was dialyzed in PBS buffer to remove excess biotin. BLI experiments were performed using an OctetRED96 instrument from PALL/ForteBio. All experiments were performed at 30 °C using PBS...
Thus, in vitro, micromolar concentrations of EN460 can inhibit ERO1α even in the presence of a vast molar excess of competing thiols. In vivo, nonselective reactivity with free thiols may explain the leftward shift in the dose-response curve (compared with the in vitro situation). ...
time (min) upon interaction with LPS micelle for Esc(1–21) (A) and Esc(1–21)-lc (B) peptides, respectively. The lower panels show corresponding enthalpy change per mole of peptide injection vs. molar ratio (peptide:LPS) for peptides Esc(1–21) and Esc(1–21)-lc, respectively. ...
Crystals of Spindlin1/C11orf84/H3K4me3K9me3 ternary complex were obtained at 291 K with the vapor diffusion hanging drop method by mixing 1:1.5 molar ratio Spindlin1 (aa: 50–262)/C11orf84 (aa: 253–295) binary complex with H3K4me3K9me3 (A1-G12) peptide in the crystallization buffer...
For crystallization of the Tat-SF1 UHM–SF3b155 ULM4 and ULM5 complexes, 0.1 mm Tat-SF1 UHM was mixed with a 1.2-fold molar excess of SF3b155 ULM and concentrated in a 2-kDa MWCO Vivaspin® Hydrosart (Sartorius) concentrator. The Tat-SF1 UHM and its ULM complexes were crystallized...
Osmolar ConcentrationSolutionsUsing Mg(2+)-selective macroelectrodes based on the neutral carriers ETH 7025 and ETH 5506, methods were developed to determine accurately the apparent binding constant (Kapp) and purity, and hence the ionized magnesium concentration ([Mg2+]), in Ca(2+)-free, Mg2+...
3D). Similarly, comparison of the binding of NBD-lipid II to the PBP1b or PBP1b-complexed with moenomycin (protein:moenomycin molar ratio 1:2) shows a higher FA signal for PBP1b:moenomycin when compared to PBP1b alone (Fig. 3E). This result can be explained on the basis of ...
The extent of drug incorporation by these novel micellar clusters is 1 order of magnitude higher than is seen with triblock copolymers, with molar polymer/drug ratios of 1:48 to 1:67. On intravenous injection, the pharmacodynamic activity of a carbohydrate propofol formulation is increased by 1 ...
M molar or molarity m multiplet Me methyl mesyl methanesulfonyl mg milligram(s) MHz megahertz (frequency) Min minute(s) mL milliliter(s) mM millimolar mmol millimole(s) mol mole(s) MS Mass spectral analysis MTBE methyl t-butyl ether N normal or normality NBS N-bromosuccinimide NCS N-chlor...
A 20.0 g (0.189 mol) quantity of benzaldehyde was added dropwise with stirring to 45.3 g (0.754 mol, 4/1 molar ratio) of ethylene diamine which had been dissolved in 500 ml methanol and the solution chilled to 0° C. After the benzaldehyde addition was complete, a 7.13 g (0.189 mol)...