Brains were dissected from P30 mice and homogenized in solubilization buffer (50 mM HEPES pH = 7.5, 150 mM NaCl, 10% glycerol, 1% Triton x-100, 1 mM EDTA pH = 8, 1 mM EGTA pH = 8, 1.5 mM MgCl2, 200 μM Na3VO4, and protease inhibitor cocktail 1 diluted 1:100 (Merck by Merc...
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