Membranes were blocked for 45 min in TBST buffer (0.05 M Tris HCl pH = 7.5, 0.15 M NaCl, and 0.1% Tween 20) with 6% skimmed milk, and blotted overnight with rat anti-MBP antibody (Merck MAB386) and rabbit anti-β-Tubulin 4 (Abcam by Zotal Ltd., Tel Aviv. Israel; AB179509) ...