Lentiviral dCas9-VPR vectors for robust gene activation in a broad range of relevant cell types Purified lentiviral particles or plasmid DNA for generation of stable dCas9-VPR nuclease-expressing cell populations Inquirefor pricing on larger volumes ...
CRISPR/Cas9 mediated endogenous gene activation (CRISPRa) using enzymatically inactive Cas9 (dCas9) fused to transcription activation domains (VPR) allows for precise and fine-tuned gene activity control when targeted to promoter or enhancer regions of interest via guide RNAs (gRNAs). Transgenic ...
将CAG-LoxP-STOP-LoxP-dCas9-VPR-IRES-EGFP-WPRE-polyA结构插入到Rosa26基因中。失活的dCas9与三元转录激活因子VPR(VP64-p65-Rta)融合,与Cre工具鼠交配后,可在有Cre表达的细胞中对目的基因进行转录激活调控,增强目的基因的表达。 应用领域:基因调控工具,基因激活,CRISPRa ...
respectively. The cell lines are optimized to work alongside Horizon’s Edit-R predesigned synthetic single guide RNA (sgRNA) and CRISPRa guide RNA, offering researchers a complete solution to simplify and streamline
Transgenic animals with increased or abrogated target gene expression are powerful tools for drug discovery research. Here, we developed a CRISPR-based Rosa26-LSL-dCas9-VPR mouse model for targeted induction of endogenous gene expression using different
We centred our approach on a dCas9-based gene activation sys- tem because with this, the activator can be targeted at one or more genomic locations easily and simultaneously simply by expressing the right combination of guide RNAs. Moreover, the functionality of the conventional CRISPR/Cas9 ...