CRISPRa dCas9-VPR lentiviral particles express a human codon-optimized version of the nuclease-deactivated Cas9 gene, fused to three transcriptional activators (VP64, p65 and Rta), and two nuclear localization signals (NLS). When paired with a well-designed guide RNA that targets a gene near ...
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Transgenic animals with increased or abrogated target gene expression are powerful tools for drug discovery research. Here, we developed a CRISPR-based Rosa26-LSL-dCas9-VPR mouse model for targeted induction of endogenous gene expression using different Adeno-associated virus (AAV) capsid variants for...
将CAG-LoxP-STOP-LoxP-dCas9-VPR-IRES-EGFP-WPRE-polyA结构插入到Rosa26基因中。失活的dCas9与三元转录激活因子VPR(VP64-p65-Rta)融合,与Cre工具鼠交配后,可在有Cre表达的细胞中对目的基因进行转录激活调控,增强目的基因的表达。 应用领域:基因调控工具,基因激活,CRISPRa ...
need to engineer a cell line specifically for this purpose. Removing the time-intensive step of generating a stable cell line and the cost associated with purchasing a nuclease could help researchers increase R&D productivity and allow novice users gain a better understanding of the CRISPR wo...
Transgenic animals with increased or abrogated target gene expression are powerful tools for drug discovery research. Here, we developed a CRISPR-based Rosa26-LSL-dCas9-VPR mouse model for targeted induction of endogenous gene expression using different