dCas9-SAM: an engine for activation the endogenous gene expression Synergistic activation mediator (SAM)is developed at the basis of CRISPR/Cas9 technique. Wildtype cas9 has been mutated at amino acid positionsD10AandH840Awhich completely ...
Generation of an inducible dCas9-SAM human PSC line for endogenous gene activationdoi:10.3389/fcell.2024.1484955Petazzi, PaoloGutierrez-Agüera, FranciscoRoca-Ho, HeleiaCastao, JulioBueno, ClaraAlvarez, NiuskaForrester, Lesley M.Sevilla, Ana
We next compared dCas9-CBP with the SAM system at other gene loci in S2 cells. We found that dCas9-CBP could weakly activate thewingless(wg) promoter, whereas the SAM system did not have a statistically significant effect on this gene (Fig.2A). By contrast, althoughenandAttacin-C (AttC...
高效特异的CRISPRdCas9转录激活系统——flySAM系统 中文名称:3,3,5,5-四甲基联苯胺盐酸盐二水合物 中文别名:3,3,5,5-四甲基苯并啶二盐酸酸水合物 英文名称:3,3,5,5-tetramethylbiphenyl-4,4-diamine hydrochloride hydrate 英文别名:TMB-2HCL 外观:白色至类白色结晶粉末 含量:≥ 99% 分子式:C16H20N2.2ClH...
dCas9-SAM: an engine for activation the endogenous gene expression Synergistic activation mediator (SAM)is developed at the basis of CRISPR/Cas9 technique. Wildtype cas9 has been mutated at amino acid positionsD10AandH840Awhich completely inactivate both the nuclease and nickase activities. The res...
1. 基因敲除/基因敲入 i. 基因修复途径介绍(NHEJ和HDR) ii. Knock-in和Knock-out的简介 iii. Knock-in策略简介(HDR/Retron/双pegRNA策略/GRAND/TJ-PE) 2. 多敲系统简介 3. CRISPRa/CRISPRi(基因激活与基因抑制) i. dCas9-PVPR系统介绍 ii. dCas9-VP64/GI/SAM基因激活系统介绍 ...
基因名称: SAMD12 Gene ID: 401474 种属 Homo sapiens DNA序列编号: NM_001101676.2 DNA描述 Homo sapiens sterile alpha motif domain containing 12 (SAMD12), transcript variant 1, mRNA DNA大小: 8875 bp DNA编码区: atggctgtggaagctctccactgtggtttgaatccacggggtattgatcaccctgcccat gctgaaggtattaaactgcaaat...
基因名称: SAMD4A 基因又名: DKFZp434H0350、SMG、SMGA、KIAA1053、Smaug、Smaug1 Gene ID: 23034 种属: Homo sapiens 基因序列编号: NM_015589 基因描述: Homo sapiens sterile alpha motif domain containing 4A (SAMD4A), transcript variant 1, mRNA 基因编码区: atgatgtttcgcgaccaggtcggggtgctggcgggctgg...
综上所述,该研究利用CRISPR/dCas9-SAM系统与高通量测序技术相结合,对胃癌差异表达的蛋白编码基因及lncRNA基因与胃癌细胞增殖的关系进行了全面筛选,获得了一批可能参与胃癌细胞增殖调控的蛋白编码基因及lncRNA基因,深入研究这些基因与胃癌细胞增殖及胃癌发生发展的关系,为深入揭示胃癌的发病机制提供新的线索。
Comprehensive off-target analysis of dCas9-SAM-mediated HIV reactivation via long noncoding RNA and mRNA profilingGenome editing, CRISPR, Off-target, RNA sequencing, Transcriptome, HIV, Latency, Shock and killBackground CRISPR/CAS9 (epi)genome editing revolutionized the field of gene and cell ...