利用wdr-5(ok1747);daf-16(mu86)双突变体,我们进一步发现WDR-5调控线虫寿命需要DAF-16的参与。而激光共聚焦和RT-qPCR的结果发现,WDR-5能够抑制DAF-16入核以及降低DAF-16靶基因的mRNA表达水平。而且该抑制作用依赖于PRMT-1。最后,我们通过co-IP(免疫共沉淀)实验,发现WDR-5抑制了PRMT-1与DAF-16的结合。
Prolongation of lifespan and induction of gene expression by luteolin under oxidative stress conditions were not seen in daf-16 (mu86) mutants. These results suggest that the enhancement of oxidative stress tolerance by luteolin was mediated by the daf-16 pathway in C....
And neither skn-1(lax120); fat -5(tm420) or daf-16(mu86); fat -5(tm420) double mutants showed a strengened weaker HS resistance ability than single mutants. These together indicated that palmitoleic acid or its derivative may be the mediator through which SKN-1 inhibited the function ...
Worm strain with daf-2(e1370) mutation presented enhanced HS-induced Cd resistance, which was eliminated in worm strains of daf-16(mu86) and hsf-1(sy441). HS pretreatment increased DAF-16 nuclear localization and HSF-1 granule formation prior to Cd exposure. DAF-16 and HSF-1 was ...
C. eleganswere cultured using standard techniques34. The strains used in this work were N2 Bristol (wild-type),DAF-16/FoxO(mu86),DAF-16/FoxO(m26),DAF-16/FoxO(mgDf50); daf-2(e1370), DAF-16/FoxO(mgDf50); daf-2(e1370)unc-119(ed3); lpIs12[DAF-16/FoxOa::RFP + unc-119(...
(A) The effect of CLJP on the lifespan of daf-16 mutant worms (mu86). Statistical results were summarized in Table 4. (B) The effect of CLJP on the mRNA expressions of aging-related genes. (C) Representative fluoresce images and quantification of DAF-16::GFP localization. Scale bar,...
Notes: survival curves of mutants under Cr (VI)-induced oxidative stress conditions: (a) eat-2 (ad465); (b) rsks-1 (ok1255); (c) isp-1 (qm150); (d) nuo-6 (qm200); (e) glp-1 (e2144); (f) daf-16 (mu86); (g) daf-2 (e1370); and (h) age-1 (hx546), ...
we performed a PSG-specific gene profiling analysis by extracting RNA from isolated PSG cells of a daf-2-reduction-of-function (rf) mutant, daf-2(e1370) (high DAF-16/FOXO activity) and a daf-16(0); daf-2(rf) double mutant with a null allele of daf-16, mu86 (no DAF-16/FOXO ...
elegans strains used in this study (wild-type N2 var. Bristol, CF1038 [daf-16(mu86) I.], TJ356 [zIs356 IV (pdaf-16-daf-16: gfp; rol-6)], VC199 sir-2.1 (ok434) IV and LD001 [ldIs007 pskn-1: skn-1b/c: gfp; rol-6]) and bacterial strains were provided by the ...
To validate our results, we determined the effect of GE on lipid accumulation using both skn-1 (mg570; GR2245) and daf-16 (mu86; CF1038) mutant strains. As can be observed in Figure 4C, treatment with our GE from L1 to L4 with the skn-1 mutant induced a significant reduction in ...