利用wdr-5(ok1747);daf-16(mu86)双突变体,我们进一步发现WDR-5调控线虫寿命需要DAF-16的参与。而激光共聚焦和RT-qPCR的结果发现,WDR-5能够抑制DAF-16入核以及降低DAF-16靶基因的mRNA表达水平。而且该抑制作用依赖于PRMT-1。最后,我们通过co-IP(免疫共沉淀)实验,发现WDR-5抑制了PRMT-1与DAF-16的结合。
C. eleganswere cultured using standard techniques34. The strains used in this work were N2 Bristol (wild-type),DAF-16/FoxO(mu86),DAF-16/FoxO(m26),DAF-16/FoxO(mgDf50); daf-2(e1370), DAF-16/FoxO(mgDf50); daf-2(e1370)unc-119(ed3); lpIs12[DAF-16/FoxOa::RFP + unc-119(...
2.1 组织特异性拯救daf-16b/c亚型对daf-16(mu86);daf-2(e1370)寿命和抗性的 影响………..55 2.1.1 肠道特异性拯救daftl6b/c对daf-16(mu86);daf-2(e1370)寿命和抗性的 影响……….55 2.1.2表皮特异性拯救daftl6b/c对daf-16(mu86);daf-2(e1370)寿命和抗性的 影响……….58 2.1.3神经系统和...
Prolongation of lifespan and induction of gene expression by luteolin under oxidative stress conditions were not seen in daf-16 (mu86) mutants. These results suggest that the enhancement of oxidative stress tolerance by luteolin was mediated by the daf-16 pathway in C. elegans....
The C. elegans strains used were N2: Bristol (wildtype), CF1038: daf-16 (mu86), CF1553: sod-3 (muIs84), and DA1116: eat-2 (ad1116), which were obtained from Shanghai Tech University. The PS3551: hsf-1 (sy441), CB1370: daf-2 (e1370), CL2070: hsp-16.2 (dvIs70), EU31...
Worm strain with daf-2(e1370) mutation presented enhanced HS-induced Cd resistance, which was eliminated in worm strains of daf-16(mu86) and hsf-1(sy441). HS pretreatment increased DAF-16 nuclear localization and HSF-1 granule formation prior to Cd exposure. DAF-16 and HSF-1 was ...
And neither skn-1(lax120); fat -5(tm420) or daf-16(mu86); fat -5(tm420) double mutants showed a strengened weaker HS resistance ability than single mutants. These together indicated that palmitoleic acid or its derivative may be the mediator through which SKN-1 inhibited the function ...
we performed a PSG-specific gene profiling analysis by extracting RNA from isolated PSG cells of a daf-2-reduction-of-function (rf) mutant, daf-2(e1370) (high DAF-16/FOXO activity) and a daf-16(0); daf-2(rf) double mutant with a null allele of daf-16, mu86 (no DAF-16/FOXO ...
c C. elegans with the genotypes wild type, daf-16(mu86lf), and hlh-30 (tm1978lf) were grown to young adulthood at 20 °C, then harvested, and their transcriptomes determined by mRNA-seq. The Venn diagrams illustrate the number of genes significantly regulated in the mutants compared to ...
To validate our results, we determined the effect of GE on lipid accumulation using both skn-1 (mg570; GR2245) and daf-16 (mu86; CF1038) mutant strains. As can be observed in Figure 4C, treatment with our GE from L1 to L4 with the skn-1 mutant induced a significant reduction in ...