利用wdr-5(ok1747);daf-16(mu86)双突变体,我们进一步发现WDR-5调控线虫寿命需要DAF-16的参与。而激光共聚焦和RT-qPCR的结果发现,WDR-5能够抑制DAF-16入核以及降低DAF-16靶基因的mRNA表达水平。而且该抑制作用依赖于PRMT-1。最后,我们通过co-IP(免疫共沉淀)实验,发现WDR-5抑制了PRMT-1与DAF-16的结合。
C. elegansstrains and culture C. eleganswere cultured using standard techniques34. The strains used in this work were N2 Bristol (wild-type),DAF-16/FoxO(mu86),DAF-16/FoxO(m26),DAF-16/FoxO(mgDf50); daf-2(e1370), DAF-16/FoxO(mgDf50); daf-2(e1370)unc-119(ed3); lpIs12[DAF-16/...
2.1 组织特异性拯救daf-16b/c亚型对daf-16(mu86);daf-2(e1370)寿命和抗性的 影响………..55 2.1.1 肠道特异性拯救daftl6b/c对daf-16(mu86);daf-2(e1370)寿命和抗性的 影响……….55 2.1.2表皮特异性拯救daftl6b/c对daf-16(mu86);daf-2(e1370)寿命和抗性的 影响……….58 2.1.3神经系统和...
The C. elegans strains used were N2: Bristol (wildtype), CF1038: daf-16 (mu86), CF1553: sod-3 (muIs84), and DA1116: eat-2 (ad1116), which were obtained from Shanghai Tech University. The PS3551: hsf-1 (sy441), CB1370: daf-2 (e1370), CL2070: hsp-16.2 (dvIs70), EU31...
Prolongation of lifespan and induction of gene expression by luteolin under oxidative stress conditions were not seen in daf-16 (mu86) mutants. These results suggest that the enhancement of oxidative stress tolerance by luteolin was mediated by the daf-16 pathway in C. elegans....
Worm strain with daf-2(e1370) mutation presented enhanced HS-induced Cd resistance, which was eliminated in worm strains of daf-16(mu86) and hsf-1(sy441). HS pretreatment increased DAF-16 nuclear localization and HSF-1 granule formation prior to Cd exposure. DAF-16 and HSF-1 was ...
And neither skn-1(lax120); fat -5(tm420) or daf-16(mu86); fat -5(tm420) double mutants showed a strengened weaker HS resistance ability than single mutants. These together indicated that palmitoleic acid or its derivative may be the mediator through which SKN-1 inhibited the function ...
we performed a PSG-specific gene profiling analysis by extracting RNA from isolated PSG cells of a daf-2-reduction-of-function (rf) mutant, daf-2(e1370) (high DAF-16/FOXO activity) and a daf-16(0); daf-2(rf) double mutant with a null allele of daf-16, mu86 (no DAF-16/FOXO ...
(mu86lf); glp-1(e2141ts) daf-16(mu86lf); glp-1(e2141ts); hlh-30(tm1978lf) 80% 60% hlh-30 loss Lifespan in glp-1 40% 20% 0% 0 daf-16 loss 5 10 15 20 25 Time (days) d Normal conditions Low insulin/ IGF signaling No germline DAF-16 HLH-30 DAF-16 HLH-30 Normal ...
To validate our results, we determined the effect of GE on lipid accumulation using both skn-1 (mg570; GR2245) and daf-16 (mu86; CF1038) mutant strains. As can be observed in Figure 4C, treatment with our GE from L1 to L4 with the skn-1 mutant induced a significant reduction in ...