酶法合成D-阿洛酮糖主要是依靠D-阿洛酮糖 3-差向异构酶(D-allulose 3-epimerase,DAEase)催化D-果糖在C3位发生异构化实现的,目前已报道的DAEase约有30种,平衡转化率基本处于24%～33%[17]。然而,传统酶法存在酶纯化步骤烦琐与产物...
这些特征在本领域中是已知的,并且参见例如Chan等.,StructuralBasisforSubstrateSpecificityinPhosphateBinding(beta/alpha)8-Barrels:D-Allulose6-Phosphate3-EpimerasefromEscherichiacoliK-12.Biochemistry.2008;47(36);9608–9617。优选地,用于本发明方法的差向异构酶含有用于催化的(α/β) 8 -桶结构域、在所述桶的...
部分原文报道如下: This assessment addresses a food enzyme preparation consisting of the immobilised non-viable cells of the non-genetically modified bacterium identified by the applicant (Samyang Corporation) as Microbacterium foliorum strain SYG27B. This strain produces the enzyme D-psicose 3-epimerase ...
The invention also relates to a process for preparing allulose where the process involves converting fructose 6-phosphate (F6P) to allulose 6-phosphate (A6P), catalyzed by allulose 6-phosphate 3-epimerase (A6PE), and converting the A6P to allulose, catalyzed by allulose 6-phosphate phosphatase...
37、阿洛酮糖-6-磷酸-3-差向异构酶(allulose-6-phosphate 3-epimerase,a6pe)用于将果糖-6-磷酸转化为阿洛酮糖-6-磷酸。在实验过程中,发明人还发现阿洛酮糖-6-磷酸-3-差向异构酶除将果糖-6-磷酸转化为阿洛酮糖-6-磷酸的作用外,还能将产物阿洛酮糖转化为果糖;若阿洛酮糖-6-磷酸-3-差向异构酶的逆反应...
As a rate-limiting enzyme,d-allulose 6-phosphate 3-epimerase fromPantoeasp. was semi-rationally engineered, and variant Y143F led to 88.8% yield ofd-allulose from 10mM sucrose using one-pot strategy. ATP consumption was reduced to 5% of the sucrose concentration after introducing ATP-...
目前阿洛酮糖的生产,采用的是生物酶催化,即采用D-塔格糖-3-异构酶(D-tagatose-3-epimerase, DTEase),包括了D-塔格糖-3-异构酶(DTEase)和D-阿洛酮糖-3-异构酶(D-psicose-3-epimerase, DPEase或D-allulose-3-epimerase, DAEase)。 D-阿洛酮糖-3-异构酶能够有效地将果糖异构化转化为阿洛酮糖,但是其大多...
目前阿洛酮糖的生产,采用的是生物酶催化,即采用D-塔格糖-3-异构酶(D-tagatose-3-epimerase, DTEase),包括了D-塔格糖-3-异构酶(DTEase)和D-阿洛酮糖-3-异构酶(D-psicose-3-epimerase, DPEase或D-allulose-3-epimerase, DAEase)。 D-阿洛酮糖-3-异构酶能够有效地将果糖异构化转化为阿洛酮糖,但是其大多...
with Potential d-allulose 3-epimrase Activity We found a putative dolichol phosphate mannose synthases (DPMS) from Bacillus sp., which exhibited the highest specific activity toward d -allulose, one of... MJ Seo,ER Kwon,JK Sang,... - 《Biotechnology & Bioprocess Engineering》 被引量: 0发表...
Organic Allulose Sweetener Sugar D-psicose 3-epimerase (dpe) D- psicoseDESCRIPTIONAllulose is a low-calorie rare sugar, that delivers the taste, texture and enjoyment of sucrose but offers 90% fewer calories with no sugar. It is approximately 70% as sweet as sucrose...