Cystine-knot peptides: Emerging tools for cancer imaging and therapy. Expert Rev. Proteomics 2014, 11, 561-572.Ackerman SE, Currier NV, Bergen JM, Cochran JR. Cystine-knot peptides: emerg- ing tools for cancer imaging and therapy. Expert Rev Proteomics. 2014;11:561-572....
Disclosed are peptides comprising a molecular scaffold portion and a loop portion that binds to integrin α v β 6 . This integrin is expressed on pancreatic tumors, making the peptides useful as imaging agents, among other uses. The peptides showed single-digit nanomolar dissociation constants ...
A truncated form of the Agouti-related protein (AgRP), a member of the cystine-knot family, has shown promise as a scaffold for engineering novel peptides with new molecular recognition properties. In this study, we replaced a constrained six amino acid loop in AgRP with a nine amino acid ...
Recently, we demonstrated that the cystine-knot peptide EETI-II is internalized into cells and that its cellular uptake could be modulated by using a protein transfection reagent Xfect. However, the mechanism of Xfect-mediated cellular internalization of EETI-II remained unclear. Here, by using ...
Advances in precision molecular imaging promise to transform our ability to detect, diagnose and treat disease. Here, we describe the engineering and validation of a new cystine knot peptide (knottin) that selectively recognizes human integrin αvβ6 wit
Interestingly one of the cystine-knotted protein families is the cyclotides, where the N and C termini are additionally linked forming a fully circularized peptide. This bond is formed between the carboxy terminal and an asparagine side chain [62] catalyzed by the enzyme asparaginyl endopeptidase [...
Phrixotoxin-1,-2 and-3 contain an Inhibitor Cystine Knot ( ICK ) motif, and thus belong to the ICK peptide family.The motif is similar to the cycpc cystine knot or cyclotide, but lacks the cycpsation of the polypeptide backbone which is present in the latter family.Ho...
99mTc-Labeled cystine-knot peptide for integrin {alpha}v{beta}6 targeted tumor imaging It is highly important to develop agents for non-invasive imaging integrin αvβ6 expression since it may provide a technique for cancer early diagnosis, prognosis and treatment planning. We recently discovered ...
Reinwarth, M., Nasu, D., Kolmar, H. and Avrutina, O. (2012) Chemical synthesis, backbone cyclization and oxidative folding of cystine-knot peptides: promising scaffolds for applications in drug design. Molecules (Basel, Switzerland) 17, 12533-12552....
Kolmar, A. Bernkop-Schnurch, The potential of cystine-knot microproteins as novel pharmacophoric scaffolds in oral peptide drug delivery, J. Drug Target. 14 (2006) 137-146.Werle M, Schmitz T, Huang HL, Wentzel A, Kolmar H, Bernkop-Schnurch A. The potential of cystine-knot micro...