pisum were correlated with a decrease of cathepsin B- and L-like proteinase activities. In the case of M. persicae, there was an increase of these proteolytic activities, but also of the aminopeptidaselike ...
Many proteinase inhibitors from plants are characterized by “double-headedness”, i.e. each molecule of such inhibitor can simultaneously bind two molecules of the same or different proteinases in two reactive centers. There are a large number of proteinase inhibitors in plants, which provides addi...
like papain, actinidin, proteinase Ω, chymopapain,stem bromelainand mexicain. Most of them belong to the papain andlegumainfamilies (Grudkowska and Zagdanska, 2004). The best-known CPs are caspase-like proteins, vacuolar-processing enzymes, papain-like peptides, and cathepsin-type proteases (Palm...
Cysteine proteases, expressed as pre-proteins, consist of an I29 inhibitor domain preventing non-specific activity [27]. In our study, transcription of the entire set of nodule VPE cysteine proteases strongly increased coinciding with the progression of senescence. VPEs are therefore predominantly ...
Evolutionary features of cystatins inT. urticae.(A) Phylogram ofT. urticaecystatin sequences showing the presence of functional conserved residues in the four cystatin groups deduced from the phylogenetic tree. Amino acid sequences responsible of the inhibitor-C1A enzyme interaction are shaded in green...
To generate models compatible with ligand binding, we modeled the proteins in the presence of the hydroxymethyl ketone inhibitor located in the PDB template. Additionally, to better evaluate the impact of residue differences on ligand binding, we determined the frequency of ligand interactions with ...
oxidative stress. Similar to GSH, Procysteine has been found to contain anti-oxidative capabilities via restoration of glutathione levels in atrophied muscles. This compound has also been found to stimulate gene expression of insulin-like growth factor-1, cardiotrophin-1, and ciliary neurotrophic ...
Poliovirus proteinase 3 C: large-scale expression, purification and specific cleavage activity on natural and synthetic substrates in vitro. J. Virol. 62, 4586–4593 (1988). CAS PubMed PubMed Central Google Scholar Han, Y. S. et al. Papain-like protease 2 (PLP2) from severe acute ...
Figure 1. IGF-IR functions are blocked in tumor cells treated with the cysteine proteinase inhibitor E-64.A, reduced anchorage-independent growth. H-59 and MCF-7 cells were cultured in semi-solid agar for 12 days in the absence (aand c) or presence (b and d) of 10 μg/ml E-64....
The LanD-like flavoproteins, belonging to the family of homo-oligomeric flavin-containing cysteine decarboxylases, catalyze the oxidative decarboxylation of 1 at the C-terminus of the peptide to form an enethiol (Fig. 18).82,83 This Cys-derived enethiol is highly nucleophilic and easily reacts...